Document 3228
 DOCN  M94A3228
 TI    DNA staining changes associated with apoptosis and necrosis in
       lymphocytes of individuals with HIV infection.
 DT    9412
 AU    Echaniz P; de Juan MD; Cuadrado E; Hosp. Ntra. Sra. de Aranzazu, San
       Sebastian, Spain.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):124 (abstract no. PA0115). Unique
       Identifier : AIDSLINE ICA10/94369347
 AB    OBJECTIVE: We report here a flow cytometry method dealing with the
       quantitation of cells which die by apoptosis or necrosis, and its uses
       in individuals at different stages of HIV infection. METHOD: The method
       uses low concentrations of two DNA binding dyes tha allow to establish
       selective regions for live, apoptotic and necrotic cells in a rat
       thymocyte model. PBLC from patients and controls were analyzed either
       freshly obtained or after activation with PWM. RESULTS: TABULAR DATA,
       SEE ABSTRACT VOLUME. Results are expressed as %1SD of cells within
       cytogram regions defining live apoptotic and necrotic cells; results of
       PWM-stimulated cultures are in brakets. DISCUSSION: Individuals with HIV
       infection show significant incrase of cell mortality atributable to
       programmed cell death. Whereas a substantial proportion of these cells
       display DNA fragmentation and DNA binding dye profiles characteristic of
       apoptosis, a significant proportion exhibit features of DNA staining
       similar to the observed when cells die by accidental death or necrosis.
 DE    Apoptosis  Comparative Study  DNA/ANALYSIS/*METABOLISM  Flow
       Cytometry/METHODS  Human  HIV Infections/BLOOD/*IMMUNOLOGY  HIV
       Seropositivity/BLOOD/*IMMUNOLOGY
       Lymphocytes/CYTOLOGY/METABOLISM/*PATHOLOGY  Necrosis  Reference Values
       Stains and Staining  MEETING ABSTRACT

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