Document 3229
 DOCN  M94A3229
 TI    Lipid peroxidation role in formation of immunodeficiency in HIV-1
       infection.
 DT    9412
 AU    Pastushenkov V; Mitin Y; Karpischenko A; Ivanov G; Military Medical
       Academy, St. Petersburg, Russia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):123 (abstract no. PA0113). Unique
       Identifier : AIDSLINE ICA10/94369346
 AB    OBJECTIVE: To study the influence of lipid peroxidation on lymphocyte
       function and nuclear chromatin degradation in the course of
       immunodeficiency progression in HIV-1 infection. METHODS: 37 HIV-1
       infection patients (WB and ELISE) persons were investigated. T and B
       cells subsets were counted by cytofluorimetric assay. Biochemical
       criteria of lymphocyte function were: activity of manthine oxidase, NAD
       and NADPH oxidase, and the enzymes of glutathione metabolism. Chromatin
       degradation products (polydexy nucleoproteides) and the intensity of DNA
       single-strand breaks were studied by spectrophotometric assay. Lipid
       peroxidation intensity was assessed by the determination of
       thiobarbituric acid--reactive substances. RESULTS: Lipid peroxidation
       intensity in HIV-1 patients' lymphocytes was significantely increased
       compared to control. Its dynamics was parallel to that of chromatin
       degradation process and inversely correlated with the parameters of
       lymphocytes function. CONCLUSIONS: Lipid peroxidation takes an important
       part in the development of lymphocyte dysfunction and, hence, of immune
       deficiency state in HIV-1 infection.
 DE    Acquired Immunodeficiency Syndrome/*BLOOD/*IMMUNOLOGY  B-Lymphocyte
       Subsets/*IMMUNOLOGY/METABOLISM  Glutathione/BLOOD  Human  *HIV-1  *Lipid
       Peroxidation  Multienzyme Complexes/BLOOD  NADH, NADPH
       Oxidoreductases/BLOOD  T-Lymphocyte Subsets/*IMMUNOLOGY/METABOLISM
       Xanthine Oxidase/BLOOD  MEETING ABSTRACT

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