Document 3232
 DOCN  M94A3232
 TI    Association between changes of HIV-1 quasispecies in serum and disease
       progression.
 DT    9412
 AU    Ida S; Oka S; Hirabayashi Y; Shioda T; Nagai Y; Kimura S; Shimada K;
       Institute of Medical Science, University of Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):123 (abstract no. PA0112). Unique
       Identifier : AIDSLINE ICA10/94369343
 AB    OBJECTIVE: In order to address the virulent variants which correlate
       with clinical deterioration, we analyzed the genetic changes of HIV-1 in
       sera obtained from three patients whose CD4 counts had declined rapidly.
       MATERIALS AND METHODS: All patients are Japanese hemophiliacs who were
       infected with HIV-1 at least before 1985. Rapid decline of CD4 counts
       was noted from 1989 to 1990 in these patients. Sera were obtained at
       three to four time points during, before and after CD4 depression. Total
       RNA extracted from 100 microliters of serum was reverse transcripted and
       amplified for envelope V3 region by the nested PCR. The amplified DNA
       was cloned and al least 10 clones were sequenced. RESULTS AND
       CONCLUSION: Fluctuations in quasispecies were observed in all cases
       during study periods. Among them, newly occurring clones having amino
       acid substitution from serine to arginine (positively charged amino
       acid) at position 11 from the first cysteine of the V3 loop were
       detected as a major clone in each patient four to six months before the
       deterioration in all cases. These clones have evolved with additive
       mutations thereafter. It might be postulated that these mutations lead
       to not only antigenic variation to escape from the host immunity but
       also adding virulency of the virus in vivo.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*MICROBIOLOGY/  PHYSIOPATHOLOGY
       Hemophilia/COMPLICATIONS  Human  HIV-1/GENETICS/*ISOLATION &
       PURIF/PATHOGENICITY  *Point Mutation  Polymerase Chain Reaction/METHODS
       RNA, Viral/*BLOOD/ISOLATION & PURIF  T4 Lymphocytes/IMMUNOLOGY  Viral
       Envelope Proteins/BIOSYNTHESIS  Virulence  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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