Document 3237
 DOCN  M94A3237
 TI    Soluble CD95 (FAS delta TM) inhibits HIV-related apoptosis.
 DT    9412
 AU    Krowka JF; Sheppard HW; Ascher MS; Fitzpatrick P; Kiefer MC; Pavloff N;
       Barr PJ; VRDL, CA Dept. of Health Services, Berkeley 94704.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):121 (abstract no. PA0105). Unique
       Identifier : AIDSLINE ICA10/94369338
 AB    OBJECTIVE: To analyze the role of the apoptosis/activation antigen FAS
       (CD95) in depletion of lymphocytes from HIV-infected (HIV+) individuals.
       METHODS: Peripheral blood lymphocytes (PBL) from HIV+ and
       HIV-seronegative (HIV) participants of the San Francisco Men's Health
       Study were stained with monoclonal antibodies and analyzed by flow
       cytometry to quantitate CD95 expression. Gel electrophoresis was used to
       analyze DNA fragmentation in PBL cultured in the presence or absence of
       a soluble recombinant form of CD95 (FAS delta TM). RESULTS: Expression
       of CD95 was significantly increased on the surfaces of both CD4+ and
       CD8+ PBL from HIV+ donors in comparison to HIV controls. Enhanced CD95
       expression on CD4+ PBL Showed a strong negative correlation with CD4+
       PBL numbers in HIV+ individuals. FAS delta TM but not control
       baculovirus extracts blocked completely DNA fragmentation of
       mitogen-stimulated PBL. CONCLUSIONS: Upregulation of CD95 expression on
       PBL occurs in HIV+ donors and may be involved in CD4+ lymphocyte
       depletion. FAS delta TM can inhibit lymphocyte apoptosis. Studies to
       determine the mechanisms by which HIV affects expression of soluble
       and/or membrane-associated CD95 are currently in progress.
 DE    Antigens, CD/*PHYSIOLOGY  Antigens, CD4/PHYSIOLOGY  Antigens,
       CD8/PHYSIOLOGY  Antigens, Surface/*BIOSYNTHESIS  *Apoptosis  Comparative
       Study  Flow Cytometry  Human  *HIV Seronegativity  HIV
       Seropositivity/*IMMUNOLOGY  Lymphocytes/*IMMUNOLOGY/MICROBIOLOGY
       Receptors, Cell Surface/*BIOSYNTHESIS  T4 Lymphocytes/MICROBIOLOGY
       Up-Regulation (Physiology)  MEETING ABSTRACT

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       protected by Copyright Law (Title 17, U.S.Code).