Document 3240
 DOCN  M94A3240
 TI    Multiple input of infectious HIV-1 is essential to single cell killing.
 DT    9412
 AU    Kuroda MJ; el-Farrash MA; Kannagi M; Harada S; Kumamoto University
       School of Medicine, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):121 (abstract no. PA0104). Unique
       Identifier : AIDSLINE ICA10/94369335
 AB    OBJECTIVE: To study the mechanism of single cell killing by HIV-1 in
       CD4+ T cell line. METHODS: MT-4 cells were infected with different moi
       (1, 10 and 30) of HIV-1 in the presence or absence of neutralizing
       monoclonal antibody 0.5 beta or dextran sulfate added soon after
       adsorption. Cell growth and viability of the infected cells were
       assessed spectrophotometrically by in situ reduction of 3-(4,5
       dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT).
       Unintegrated and integrated DNA were analysed by Southern blott.
       RESULTS: Efficient blocking of HIV-1-induced single cell killing and
       establishment of persistently infected MT-4 cells were obtained in the
       presence of 0.5 beta or dextran sulfate when infected with moi 1 but not
       with higher moi of 10 to 30. The amount of unintegrated DNA, integrated
       proviral DNA and protein syntesis were parallel to viral input dose.
       CONCLUSION: Multiple internalization of virus particles is the first
       step for HIV-1-induced single cell killing. Not only the accumulation of
       unintegrated DNA but also integrated proviral DNA and viral protein
       synthesis are accompanied with the cytocidal dose of virus. The high
       amount of CD4 molecule on cell membrane may be requisite for cell
       killing.
 DE    Cell Division  Cell Line  Cell Survival  Cells, Cultured  Human
       HIV-1/GENETICS/*PHYSIOLOGY  T4 Lymphocytes/CYTOLOGY/*MICROBIOLOGY  Viral
       Proteins/BIOSYNTHESIS  Virus Integration  MEETING ABSTRACT

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