Document 3244
 DOCN  M94A3244
 TI    Acquired specific cytotoxic activity in CD8+ HIV-1IIIB carrier cell
       clones derived from CD4+CD8+ parental human T cell leukemia cell lines.
 DT    9412
 AU    Minowada J; Otani T; Kamiya T; Fujisaki Cell Center, Okayama, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):120 (abstract no. PA0100). Unique
       Identifier : AIDSLINE ICA10/94369331
 AB    OBJECTIVE: To determine the functional activity of CD8 positive HIV-1
       carrier cell clones. METHOD: A 4-hr two-color flow cytometric
       cytotoxicity assay was used. The effector/target cell ratio analyzed was
       in the 0.2-3.0/1 range. RESULTS, DISCUSSION AND CONCLUSION: Seven
       HIV-1IIIB carrier CD8+ MOLT-4 clones from T-cell leukemia cell line
       MOLT-4 with a CD4+CD8+ phenotype, and 5 CD8+ HIV-1 carrier cell lines
       from 3 other CD4+CD8+ leukemia cell lines were used. Of the 7 MOLT-4/HIV
       clones used, 4 exhibited cytotoxic activity specific to 2 immature T
       cell lines, HPB-ALL and SUP-T1, of 21 clonal leukemia cell lines tested
       with T-, B- or myelomonocytic origins. Pretreatment of the cytotoxic,
       but not the noncytotoxic, clones with PMA augmented this specific
       cytotoxic activity. Two-color cytotoxicity analysis indicated
       unidirectional cytotoxicity; (i.e. CD8+-CD4+/CD8+). While no correlation
       was found between cytotoxicity and 1) persistent infectious virus
       release, 2) gp120 antigen or 3) p24 antigen expression, CD8 positivity
       and its enhanced expression in the HIV-carrier clones appear to be
       associated with specific cytotoxicity. The in vivo target cell
       counterpart and the specific recognition molecules on the CD8-positive
       HIV-1 carrier cells remain to be determined. The observation, however,
       provides evidence for a mechanism underlying the immunodysfunction
       associated with AIDS pathogenesis.
 DE    Antigens, CD/*IMMUNOLOGY  Antigens, CD4/*IMMUNOLOGY  Antigens,
       CD8/*IMMUNOLOGY  Cell Line  Clone Cells  Comparative Study
       *Cytotoxicity, Immunologic  Flow Cytometry/METHODS  Human  HIV Core
       Protein p24/ANALYSIS/BIOSYNTHESIS  HIV Envelope Protein
       gp120/ANALYSIS/BIOSYNTHESIS  HIV-1/*IMMUNOLOGY/METABOLISM  Leukemia,
       T-Cell  Tumor Cells, Cultured  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).