Document 3249
 DOCN  M94A3249
 TI    Surface expression of p17-gag protein during HIV-1 reactivation.
 DT    9412
 AU    Yoshimura K; Matsushita S; Matsumi S; Obaru K; Takatsuki K; Second
       Department of Internal Medicine, Kumamoto University; School of
       Medicine, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):119 (abstract no. PA0094). Unique
       Identifier : AIDSLINE ICA10/94369326
 AB    OBJECTIVE: The purpose of this study was to investigate the surface
       expression of HIV-1 antigens on latently infected cell lines during
       reactivation. MATERIALS AND METHODS: CEM/LAV1 and H9/IIIB were used as
       the chronically infected cell lines. ACH-2 (T-cell line), OM-10.1
       (myeloid cell line), and U1 (promonocyte cell line) cells were used as
       the latently infected cell lines. The cell surface expression of the HIV
       proteines with or without stimulation were detected by FACS analysis and
       radioimmunoprecipitation assay (RIPA). To further investigate the p17
       antigen expression during reactivation, three different anti-p17
       mAbs(52E5,LG20,V17) was used. The epitope recognized by the mAb was
       mapped by ELISA or Western blotting assay using synthetic or recombinant
       antigens. RESULTS: Surface expression of p17 MA as well as gp120 was
       observed on the ACH-2 cells by stimulating the cells with PMA or
       TNF-alpha. Expression of gp120 was detected on OM-10.1 but not U1 cells
       with or without stimulation. Surface expression of other viral proteins
       was not observed with two cell lines on stimulation. On both H9/IIIB and
       CEM/LAV1 cells gp120 expression was detected but the expression of p17
       was not observed. Using three different mAbs(52E5,LG20,V17) we tried to
       detect the p17 antigen that was expressed on the cell surface in an
       epitope level. 52E5 but not LG20 and V17 reacted with the cell surface
       of ACH-2 cells on stimulation. 52E5 recognized the amino acids 111 to
       123 of p17, V17 and LG20 recognized the amino acids 122 to 132 of p17.
       DISCUSSION AND CONCLUSION: These results taken together suggest that the
       paticular region of p17 protein may be preferentially expressed on the
       surface of HIV infected cells in the course of viral-reactivation.
 DE    Cell Line  Cell Membrane/METABOLISM  Gene Products,
       gag/ANALYSIS/*BIOSYNTHESIS  Human  HIV Antigens/ANALYSIS/*BIOSYNTHESIS
       HIV-1/*GROWTH & DEVELOPMENT/METABOLISM  Radioimmunoprecipitation Assay
       Tumor Cells, Cultured  *Virus Activation  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).