Document 3253
 DOCN  M94A3253
 TI    Variation in susceptibility to HIV-1 & 2 of PBMCs from healthy
       Bangladeshi, and correlation with HLA type.
 DT    9412
 AU    al-Jabri AA; Taylor M; McCloskey D; Sefton A; Race E; Navarrete C;
       Oxford JS; Dept. Med. Microbiology, London Hospital Medical College,;
       University of London, U.K.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):118 (abstract no. PA0090). Unique
       Identifier : AIDSLINE ICA10/94369322
 AB    We describe an in vitro study, within a specific ethnic group, where the
       association between the HLA type and susceptibility/resistance to HIV
       infection is investigated. PBMCs from healthy Bangladeshi male subjects
       were infected with 4 different isolates of HIV-1 & 2 (an HIV-1 clinical
       isolate, HIV-1 MN, HIV-2 CBL20, and HIV-2 ROD). Ten fold dilutions (from
       10(-1) to 10(-7) of each isolate were used to infect the PBMCs. Cultures
       of PBMCs were maintained for 21 days with daily inspection for
       cytopathic effects and syncytia formation. Viral p24 levels were
       determined on days 5, 14, and 21 after infection. PBMCs from each
       subject were HLA typed by standard methods. The results obtained for the
       26 male subjects studied so far showed considerable variation in the
       replication of the different isolates and in the cytopathic effects
       obtained. No PBMCs showed complete resistance to all 4 viral isolates
       tested. However, some PBMCs showed some resistance to infection by
       certain isolates as shown by an absence of both p24 core antigen and
       syncytia formation during the 3 weeks in culture. No single HLA allele,
       has been significantly associated with susceptibility/resistance to
       infection in vitro to date. We are now performing an epitope analysis,
       and the investigation is extended to include more subjects and
       particularly females from the same ethnic group.
 DE    Alleles  Bangladesh  Comparative Study  Cytopathogenic Effect,
       Viral/IMMUNOLOGY  Human  HIV Infections/*ETIOLOGY/*IMMUNOLOGY
       HIV-1/IMMUNOLOGY/PHYSIOLOGY/*PATHOGENICITY
       HIV-2/IMMUNOLOGY/PHYSIOLOGY/*PATHOGENICITY  *HLA Antigens/GENETICS  In
       Vitro  Leukocytes, Mononuclear/IMMUNOLOGY/MICROBIOLOGY  Male  Virus
       Replication/IMMUNOLOGY  MEETING ABSTRACT

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