Document 3255
 DOCN  M94A3255
 TI    Difference in surface structure between mature and defective HIV virus
       particles.
 DT    9412
 AU    Morita C; Ashina T; Sukenaga A; Ikuta K; Goto T; Nakai M; Department of
       Microbiology, Osaka Medical College, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):118 (abstract no. PA0093). Unique
       Identifier : AIDSLINE ICA10/94369320
 AB    OBJECTIVE: Surface projections(knobs) are essential for binding HIV-1 to
       target cells, and spontaneous loss of them reduces infectivity of HIV-1.
       Our objective is to clarify the structure difference of surface
       projections between mature and defective particles. METHOD: Persistently
       HIV-1 infected Molt-4 and infected MT4 cell derived subclone L-2 were
       used. L-2 produced defective doughnut-shaped HIV-1 particles (K. Ohki et
       al. J. AIDS 4:1233-1240, 1991). These structures were investigated by
       TEM, SEM and STM. TEM specimens were examined after the staining of
       envelope glycoproteins with either tannic acid or ruthenium red. RESULT:
       The size of the mature particle showed a narrow range of diameter
       included knobs with a mean of 122 nm. This size is similar to the SEM
       data. On the other hand L-2 particles reveal a large variation with a
       mean of 139 nm. The size distributions of the knob on mature and
       defective virions are 17 nm and 11 nm respectively. Mature particles are
       smaller than defective particles, but the size of knobs is larger than
       L-2 ones. DISCUSSION AND CONCLUSION: We have extended the above study by
       using ruthenium red techniques. This can be useful for studying the
       projection structure. Defective particles had the small size of their
       surface knobs, suggesting the low yield of infectivity.
 DE    Cell Line  Comparative Study  Defective Viruses/GROWTH &
       DEVELOPMENT/PATHOGENICITY/  *ULTRASTRUCTURE  Human  HIV-1/GROWTH &
       DEVELOPMENT/PATHOGENICITY/*ULTRASTRUCTURE  Microscopy, Electron
       Microscopy, Electron, Scanning  Microscopy, Electron, Scanning
       Transmission  Particle Size  Ruthenium Red  Viral Envelope
       Proteins/ULTRASTRUCTURE  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).