Document 3256
 DOCN  M94A3256
 TI    Amplification of viral replication relevantly with reverse
       differentiation in HIV-1 infected cells with macrophage phenotype.
 DT    9412
 AU    Okada Y; Kameoka M; Kimura T; Kishi M; Ikuta K; Institute of
       Immunological Science, Hokkaido University, Sapporo,; Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):118 (abstract no. PA0091). Unique
       Identifier : AIDSLINE ICA10/94369319
 AB    OBJECTIVE: The cells of monocyte-macrophage lineage was important as
       HIV-1 reservoir, especially for understanding the persistent mechanism
       in asymptomatic carriers. Here, we examined the HIV-1 life cycle after
       the infection of differentially differentiated cells. METHODS: U937
       cells were differentially differentiated by varying dose of PMA for 3
       days, then infected with HIV-1. Thereafter, the cells were sequentially
       examined for cell viability, morphology, expression of differentiation
       antigens, superoxide generation, and HIV-1 phenotypes. RESULTS: The
       PMA-treated cells were dose-dependently differentiated to adhered
       morphology. HIV-1 replication after infection in these cells reversely
       correlated with PMA dosages, i.e. the replication rate was increasingly
       higher with lower dose of PMA. Thereafter, reverse differentiation
       (retro-differentiation) of these cells from adhered to floating
       morphology led to amplification of viral replication. Cells that were
       well differentiated with higher dose of PMA were hard to indue the
       retro-differentiation. DISCUSSION AND CONCLUSION: These results indicate
       a close correlation of HIV-1 amplification and cell differentiation
       level in the monocyte-macrophage lineage. The retro-differentiation
       phenomenon in the infected cells seems to be particularly important for
       understanding viral activation mechanism in reservoir cells. Intrinsic
       redox regulation or cytokines induced by opportunistic infections may
       involve in the stimulation of the cells.
 DE    Cell Differentiation/DRUG EFFECTS  Cell Line  Dose-Response
       Relationship, Drug  Human  HIV Infections/*MICROBIOLOGY/PATHOLOGY
       HIV-1/GROWTH & DEVELOPMENT/*PHYSIOLOGY  Macrophages/DRUG
       EFFECTS/*MICROBIOLOGY/PATHOLOGY  Monocytes/DRUG
       EFFECTS/MICROBIOLOGY/PATHOLOGY  Phenotype  Tetradecanoylphorbol
       Acetate/ADMINISTRATION & DOSAGE/PHARMACOLOGY  *Virus Replication/DRUG
       EFFECTS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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