Document 3257
 DOCN  M94A3257
 TI    Relationship between infectious HIV-1 viral load, CD4 count and clinical
       status.
 DT    9412
 AU    Margolick J; Farzadegan H; Hoover D; Rubb S; Scott E; Saah A; Johns
       Hopkins University, Baltimore, MD 21205.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):117 (abstract no. PA0089). Unique
       Identifier : AIDSLINE ICA10/94369318
 AB    OBJECTIVE: To define the interaction between infectious viral load and
       the host immune system. METHOD: 246 HIV+ homosexual men in the Johns
       Hopkins Center of the Multicenter AIDS Cohort Study (MACS) were studied.
       The 1987 clinical AIDS definition was used. Infectious units of HIV-1
       per one million PBMC (IUPM) was determined by serial 5-fold dilutions of
       10(6) PBMC, cocultured with PHA-P stimulated normal PBMC. Two color flow
       cytometry was used for analysis of lymphocyte phenotypes. RESULTS: HIV-1
       infectious viral load in PBMC was distributed log normally. IUPM was
       detectable in 194 of 246 subjects (79%) at baseline. The overall
       geometric mean was 31 IUPM. Infectious viral load was inversely related
       to the level of circulating CD4+ lymphocytes and many other lymphocytes
       subsets. No correlation was found with NK cells, B-cells or gamma-delta
       T cells. Multivariate stepwise regression analysis showed no significant
       correlation between CD38+, CD29 bright and HLA-DR+ among CD4+
       lymphocytes, but CD8+ lymphocytes expressing CD38 were independently
       associated with IUPM. CONCLUSION: Over a broad range of CD4 lymphocyte
       counts an inverse relationship between IUPM and CD4+ exists. Observed
       lower IUPM in AIDS cases after controlling for CD4 count may suggest
       that CD4+ lymphocytes in AIDS patients are less permissive for HIV-1
       replication.
 DE    Antigens, CD8/METABOLISM  Antigens, Differentiation/METABOLISM  Human
       HIV Infections/*BLOOD/IMMUNOLOGY/*MICROBIOLOGY
       *HIV-1/IMMUNOLOGY/ISOLATION & PURIF/PHYSIOLOGY  Leukocyte Count
       Lymphocyte Subsets/IMMUNOLOGY  Male  *T4 Lymphocytes/IMMUNOLOGY  Virus
       Replication/IMMUNOLOGY  MEETING ABSTRACT

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