Document 3258
 DOCN  M94A3258
 TI    Phenotypic heterogeneity between peripheral blood (PB) and lymph node
       (LN) HIV isolates.
 DT    9412
 AU    Tamalet C; Lafeuillade A; Vignoli C; Rousseau S; DeMicco P; Department
       of Virology, TimoneHospital, Marseille, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):117 (abstract no. PA0086). Unique
       Identifier : AIDSLINE ICA10/94369317
 AB    OBJECTIVE: Previous results obtained by PCR or coculture techniques have
       shown higher viral burden in lymph node mononuclear cells (LNMCs) than
       in peripheral blood mononuclear cells(PBMCs). The aim of this study was
       to comparate the phenotype of HIV simultaneously isolated from PB and LN
       in HIV infected patients (pts). METHODS: Patients :11 HIV-1 and 1 HIV-2
       infected adult pts(CDC I: 1, CDC III: 8, CDCIVC2: 3). 9 untreated pts, 3
       on Zidovudine (ZDV) or ddI therapy. Cells:PBMCs and LNMCs from LN
       surgically excised for diagnostic purposes were obtained on the same
       day. Virological studies: PB and LNMC load:ANRS consensus coculture
       protocols performed on fresh samples. In vitro sensitivity to ZDV:
       cell-free isolate sensitivity assay (ANRS consensus technique). SI
       phenotype of isolates assayed on PBMCs and MT-2 cells. RESULTS:
       Frequency of infected cells was one log greater in LNMCs compared to
       PBMCs in the 12 pts investigated. PBMC and LN isolates recovered from
       the 9 ZDV naive pts were sensitive to ZDV with similar IC50 and IC90
       values. PBMC and LN isolates from 2 pts were ZDV sensitive after at
       least 12 months of ZDV therapy. LN isolate from one IVC2 pt who had
       switched one year before to ddI after 12 months of ZDV therapy, showed a
       five-fold increase in IC90 values contrasting with an IC50 of ZDV
       sensitive isolate. SI phenotype was found in LN isolates from 2 CDCIVC2
       pts and 1 CDC III pt, while 9 LN isolates and all the 12 PBMC isolates
       were NSI. CONCLUSION: The presence in the only lymph nodes of isolates
       showing either reduced sensitivity to ZDV or SI phenotype suggests a
       greater heterogeneity of LN isolates compared to PBMC isolates and these
       data could contribute to our understanding of pathogenesis of HIV
       infection.
 DE    Adult  Comparative Study  Didanosine/THERAPEUTIC USE  Drug Resistance,
       Microbial  Human  HIV Infections/DRUG THERAPY/ETIOLOGY/*MICROBIOLOGY
       HIV-1/DRUG EFFECTS/GENETICS/*ISOLATION & PURIF  HIV-2/DRUG
       EFFECTS/GENETICS/*ISOLATION & PURIF  In Vitro  Leukocytes,
       Mononuclear/*MICROBIOLOGY  Lymph Nodes/*MICROBIOLOGY  Microbial
       Sensitivity Tests  Phenotype  Zidovudine/THERAPEUTIC USE  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).