Document 3261
 DOCN  M94A3261
 TI    V3 region sequences from HIV-1 isolates from India.
 DT    9412
 AU    Tripathy S; Renjifo B; McLane MF; Tripathy SP; Essex M; Dept. of Cancer
       Biology, Harvard School of Public Health, Boston,; Ma 02115.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):116 (abstract no. PA0084). Unique
       Identifier : AIDSLINE ICA10/94369314
 AB    AIM: To determine the V3 region sequences from HIV-1 isolates from
       heterosexually infected patients from India. METHODOLOGY: PBL samples
       were cocultured with normal PBLs and the DNA was extracted for PCR. The
       1.5 kb gp120 fragment was cloned and the V3 region sequenced. RESULTS:
       All isolates belonged to the C subtype. Most isolates had amino-acid
       residues similar to the V3 loop consensus sequences published in the Los
       Alamos database (Nov '93 III-71). One isolate had a serine in the 6 th
       position of the V3 loop. Another isolate had an arginine in the 1st
       position, alanine in the 20 th position and a phenylalanine in the 22 nd
       position of the V3 loop. These residues have not so far been reported
       for the C subtype in the Los Alamos database. The N-linked glycosylation
       site proximal to the first cysteine residue of the V3 loop was absent in
       all the isolates. CONCLUSION: C subtype of HIV-1 was the strain
       transmitted through the heterosexual route in the HIV-1 isolates
       obtained from India.
 DE    Amino Acid Sequence  DNA, Viral/BLOOD/GENETICS  Glycosylation  Human
       HIV Envelope Protein gp120/CHEMISTRY/*GENETICS  HIV
       Infections/*MICROBIOLOGY/TRANSMISSION
       HIV-1/CLASSIFICATION/*GENETICS/*ISOLATION & PURIF  India  Leukocytes,
       Mononuclear  Peptide Fragments/CHEMISTRY/*GENETICS  Sex Behavior
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).