Document 3264
 DOCN  M94A3264
 TI    Evidence for the presence of North American type HIV-I in Hyderabad,
       India.
 DT    9412
 AU    Rao R; Baskar P; Ray S; Quinn T; Hildreth JE; Bollinger R; Apollo
       Hospital, Hyderabad, India.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):116 (abstract no. PA0082). Unique
       Identifier : AIDSLINE ICA10/94369311
 AB    OBJECTIVE: To analyse the strains from Southern part of India. METHODS:
       1. HIV-1 envelope DNA was amplified by nested polymerase Chain Reaction
       from the peripheral blood mononuclear cells of HIV-1 infected
       individuals. 2. Amplified DNA was sequenced using automated flourescein
       based sequencing system. RESULTS: 1. 4 out of 6 samples sequences were
       obtained. The predicted aminoacid sequences for the V3 region are
       presented. 2. The tetrapeptide sequence GPGR, located at the crown of V3
       loop is conserved in all 4 specimens. (Fig. 1) DISCUSSION: Our analysis
       of PCR amplified envelope sequences suggests the HIV from these patients
       are homologous to North American strains. 2. This is incontrast to
       previous reports which suggest that strains from India are highly
       divergent from North America and African strains. TABULAR DATA, SEE
       ABSTRACT VOLUME.
 DE    Amino Acid Sequence  Comparative Study  Conserved Sequence  DNA,
       Viral/GENETICS  Human  HIV Envelope Protein gp120/GENETICS  HIV
       Infections/EPIDEMIOLOGY/*MICROBIOLOGY
       HIV-1/CLASSIFICATION/*GENETICS/*ISOLATION & PURIF  India/EPIDEMIOLOGY
       Molecular Sequence Data  North America/EPIDEMIOLOGY  Peptide
       Fragments/GENETICS  Polymerase Chain Reaction  Sequence Homology, Amino
       Acid  Species Specificity  MEETING ABSTRACT

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       protected by Copyright Law (Title 17, U.S.Code).