Document 3265
 DOCN  M94A3265
 TI    Serodiagnostic profiles of HIV-1 from Nigeria.
 DT    9412
 AU    Offor E; Mclane MF; Trujillo R; Kanki P; Essex M; Dept. Cancer Biology,
       Harvard School of Public Health, Boston, MA; 02115.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):115 (abstract no. PA0078). Unique
       Identifier : AIDSLINE ICA10/94369310
 AB    OBJECTIVE: To compare the serum antibody profiles of a Nigerian HIV-1
       viral isolate with those of an African (Congo) and a North American
       isolate (USA). METHOD: Three HIV-1 antigens, USA type IIIB, a Congolese
       isolate and a Nigerian isolate (N-4), were fractionated by sodium
       dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a
       nitrocellulose membrane. N-4 was isolated from peripheral blood
       lymphocytes from a Nigerian AIDS patient. Sera from 75 HIV-1 positive
       Nigerians were reacted with each of the HIV-1 strains on Western blot.
       Radioimmunoprecipitation analysis (RIPA) was performed using a positive
       T cell lysate of N-4 and a negative T cell lysate (control) to
       demonstrate the envelope and core specific bands. RESULTS: A majority of
       sera showed reactivity to Gag and Pol gene products of all three
       isolates. A few positive sera reacted with all bands, including gp120
       and gp160. Reactivity to HIV-1 gp120 was confirmed by RIPA on N-4.
       Cross-reactivity was observed between IIIB and some Nigerian sera.
       CONCLUSION: The Nigerian isolate had relatively similar antibody
       reactivity to the Congolese strain. It appears to be a prototype African
       HIV-1 isolate. Further characterization of the virus and the observed
       cross reactivity is ongoing at this time.
 DE    AIDS Serodiagnosis  Comparative Study  Congo  Cross Reactions  Gene
       Products, env/IMMUNOLOGY  Gene Products, gag/IMMUNOLOGY  Gene Products,
       pol/IMMUNOLOGY  Human  HIV Antibodies/*BLOOD  HIV Envelope Protein
       gp120/IMMUNOLOGY  HIV Infections/*IMMUNOLOGY/MICROBIOLOGY
       HIV-1/*IMMUNOLOGY/ISOLATION & PURIF  Lymphocytes/MICROBIOLOGY  Nigeria
       Protein Precursors/IMMUNOLOGY  Species Specificity  United States
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).