Document 3269
 DOCN  M94A3269
 TI    Genetic heterogeneity in vif and vpr regions of HIV-1 in PBMC from
       asymptomatic carriers.
 DT    9412
 AU    Kishi M; Zheng YH; Tokunaga K; Bahmani MK; Tateno M; Tateno Y; Oka S;
       Kurata T; Kakinuma M; Ikuta K; Inst. Immunol. Sci., Hokkaido Univ.,
       Sapporo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):114 (abstract no. PA0074). Unique
       Identifier : AIDSLINE ICA10/94369306
 AB    OBJECTIVE: MT-4 cells survived the infection with vif, vpr, or vpu
       mutant HIV-1 after transient cytopathicity, suggesting the occurrence of
       additional mutation(s) during viral replication. Here, we examined
       naturally occurring additional mutations in accessory genes essential
       for lead to persistent infection. In addition, similar mutations in
       HIV-1 in PBMC derived from asymptomatic carriers (AC) were also
       examined. METHODS: Four regions of viral genome, gag, pol endo. to vpr,
       5'-half of vif, or env (encompassing the third variable region), were
       amplified by PCR, cloned, then sequenced. RESULTS: The survivors
       producing infectious, but non-cytopathic, HIV-1 were found to carry
       heterogeneous HIV-1 genomes containing marked internal deletions in the
       region overlapping vif and vpr and nucleotide substitutions in vif
       and/or vpr region. Similar structures were also observed in PBMC
       sequentially (every year for 3 years) obtained from two AC. In addition,
       PCR analyses of serially diluted PBMC-DNAs from 93 HIV-1 carriers showed
       that only 33% in the gag-positive DNAs was positive for the region
       overlapping vif and vpr. DISCUSSION AND CONCLUSIONS: The mutations at
       vif and/or vpr were often observed in persistentently infected cells and
       PBMC from AC. Therefore, these mutations might be important for
       reduction of HIV-1 cytopathicity leading to its persistent or latent
       infection.
 DE    Cell Line  Genes, env  Genes, gag  Genes, pol  *Genes, vif  *Genes, vpr
       Human  HIV Infections/*MICROBIOLOGY  HIV-1/*GENETICS/ISOLATION & PURIF
       Leukocytes, Mononuclear/MICROBIOLOGY  Mutation  Polymerase Chain
       Reaction  Sequence Deletion  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).