Document 3270
 DOCN  M94A3270
 TI    Conserved domains of the HIV-1 vif gene in vivo.
 DT    9412
 AU    Wieland U; Seelhoff A; Salzberger B; Schwander S; Dietrich M; Eggers HJ;
       Kuehn JE; Institut f. Virologie, Universitaet Koeln, Germany.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):114 (abstract no. PA0075). Unique
       Identifier : AIDSLINE ICA10/94369305
 AB    OBJECTIVE: Determination of the genetic variability of the HIV-1 vif
       gene and identification of conserved and possibly functionally important
       regions of the complete vif gene of European and African HIV-1-infected
       patients. METHODS: PCR products of peripheral blood mononuclear cells
       extracts of 85 HIV-1 positive patients from Germany and Uganda were
       analysed by direct solid phase sequencing and temperature gradient
       gelelectrophoresis. RESULTS AND DISCUSSION: Vif consensus sequences were
       obtained for the European (proviral DNA: 58% conserved; deduced amino
       acid sequence 45% conserved) and African samples showing conserved,
       variable and hypervariable regions of the vif gene in vivo. The vif gene
       was found to be less conserved than previously thougt, with its 5' part
       being less variable than its 3' part. In sequential samples from
       individual patients the HIV-1 gene changed with the course of disease.
       However, in all patients a complete vif gene was found, indicating its
       significance in vivo. It remains to be determined which of the conserved
       motifs are essential for the function of the variable vif gene product.
 DE    Amino Acid Sequence  Base Sequence  *Conserved Sequence  DNA,
       Viral/GENETICS  *Genes, vif  Germany  Human  HIV
       Infections/*MICROBIOLOGY  HIV-1/*GENETICS/ISOLATION & PURIF
       Proviruses/GENETICS  Uganda  Variation (Genetics)  MEETING ABSTRACT

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