Document 3271
 DOCN  M94A3271
 TI    Biological properties of HIV-1 strains occurring in the Ukraine.
 DT    9412
 AU    Antonenko S; Barbasheva E; Green E; Lujlchujk M; Persidsky Y; Frolov A;
       Scientific Research Institute of Epidemiology and Infectious; Diseases,
       Kiev, Ukraine.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):114 (abstract no. PA0077). Unique
       Identifier : AIDSLINE ICA10/94369304
 AB    OBJECTIVE: To study biological properties of HIV isolated from a viruses
       carrier in the Ukraine. METHODS: Fluorescent antibody technique, ELISA
       of HIV-1 p24 antigen of the culture supernatant, RT-activity, electron
       microscopy, FCR, immunohistochemical characterization of native Nef from
       diverse isolates, using a panel of monoclonal antibodies against
       recombinant HIV-1 Nef protein (was a gift from Dr. V. Ovod, University
       of Tampere, Finland). RESULTS: 23 HIV-1 strains were isolated from
       peripheral blood lymphocytes of 45 HIV-infected persons in different
       regions of the Ukraine. 23 isolates of HIV-1 belonged to the slow/low
       type and had a low and unstable replication potential in MT-4 cell line.
       Long-term propagation of this strains did not result in any increase of
       their replicative activity. 7 isolates was thought to the rapid/high
       type and had a high infectivity, broad spectrum of cell tropism (MT-4,
       H-9, U-937, CEM cells), high levels of RT and F24 HIV antigen. The
       capacity to form syncytia was found not only in the rapid/high type
       strains but also in the majority of slow/low strains. A difference is
       detected in the immunohistochemical reactivity of murine monoclonal
       antibodies with Nef protein expressed by different isolates in MT-4
       cells. CONCLUSIONS: Both variants of HIV-1 reported in the literature:
       slow/low and rapid/high types were detected among the strains isolated
       from citizens of the Ukraine with asymptomatic infections and with
       clinical manifestations of disease.
 DE    Antibodies, Monoclonal  Gene Products, nef/IMMUNOLOGY  Human  HIV
       Antibodies  HIV Core Protein p24/METABOLISM  HIV
       Infections/*MICROBIOLOGY  HIV-1/IMMUNOLOGY/*ISOLATION & PURIF/PHYSIOLOGY
       Immunohistochemistry  Lymphocytes/MICROBIOLOGY  Reverse
       Transcriptase/METABOLISM  Ukraine  Virus Replication  MEETING ABSTRACT

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