Document 3274
 DOCN  M94A3274
 TI    Increasing persistent infection of serial passages of HIV-1 is
       correlated with mutations at vif and vpr regions.
 DT    9412
 AU    Nakaya T; Fujinaga K; Nishino Y; Kishi M; Ikuta K; Section of Serology,
       Hokkaido University, Sapporo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):113 (abstract no. PA0073). Unique
       Identifier : AIDSLINE ICA10/94369301
 AB    OBJECTIVE: We have shown that MT-4 cells survived the infection with
       vif-, vpr-, or vpu-mutant HIV-1, but not with wild-type or nef-mutant
       HIV-1. However, increasing persistent infection in the cells were
       observed by serial passage of wild-type HIV-1, but not by serial passage
       of nef-mutant HIV-1 even after 50 passages. Here, we focused on the
       naturally occurring genome mutations in the serial passages of wild-type
       HIV-1. METHODS: Transfection products of infectious molecular clone
       pNL432 and pNL-Xh (inserting a frame-shift mutation of 4 bases at the
       Xho I site in pNL432) were used as wild-type and nef-mutant HIV-1,
       respectively. Serial passages of these viruses were repeated up to 50
       times and were designated as wt-1 to wt-50 and nef(-)-1 to nef(-)-50,
       respectively. The PCR products in infected cells were cloned, then
       sequenced. RESULTS: All DNA clones derived from PCR products of the
       cells persistently infected with wt-20 and wt-50 contained termination
       codon in vpr. Substitution in vif gene was also observed in some DNA
       clones. Especially, a large deletion within vif to vpr genes was
       identified in some DNA clones. These structures were also identified in
       the M10 cells acutely infected with serial passages of wild-type, but
       not nef-mutant HIV-1. DISCUSSION AND CONCLUSIONS: The vif and vpr gene
       mutations essential for persistent infection were progressively induced
       by serial passage of wild-type HIV-1. The nef gene might be important
       for such mutations induced during the serial passage.
 DE    Codon/GENETICS  DNA, Viral/GENETICS  Frameshift Mutation  Genes, nef
       *Genes, vif  *Genes, vpr  Genes, vpu  Human  HIV
       Infections/*MICROBIOLOGY  HIV-1/*GENETICS  In Vitro  *Mutation
       Polymerase Chain Reaction  Transfection  Virus Cultivation  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).