Document 3276
 DOCN  M94A3276
 TI    An analysis of the V3 gene of HIV-1 isolated in Uganda.
 DT    9412
 AU    Mitarai S; Byarugaba W; Oishi K; Nagatake T; Matsumoto K; Fukasawa M;
       Department of Internal Medicine, Nagasaki University, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):113 (abstract no. PA0071). Unique
       Identifier : AIDSLINE ICA10/94369299
 AB    OBJECTIVE: To isolate the most reproductive HIV-1 strain from Ugandan
       HIV-1 infected patients and determine its genotype with the V3 loop
       sequence. METHODS: Peripheral blood samples were collected from the
       HIV-1 seropositive patients at Mulago hospital in 1991. The patients
       were one male and 5 female, and the mean age was 29.7. Viral RNA were
       extracted from the serum and reverse trascriptated. The 650 base pairs
       of env gene including V3 region was amplified by the nested PCR method.
       The PCR product was cloned into plasmid vector and sequenced. RESULTS:
       All of the strains obtained from the patients have same amino acid
       sequence GPGRAFV at the apex of the V3 loop. They have some mismatches
       around the apex region, but they are similar to the American European
       type and are different from the old African type already reported.
       DISCUSSION AND CONCLUSIONS: We assume these strains may be imported from
       the neiboring countries or a result of natural immunological selection.
       In order to know the reason of type variation, further investigation
       should be needed.
 DE    Adult  Amino Acid Sequence  Female  *Genes, env  Genotype  Human  HIV
       Envelope Protein gp120/*GENETICS  HIV Infections/*MICROBIOLOGY
       HIV-1/*GENETICS/ISOLATION & PURIF  Male  Molecular Sequence Data
       Peptide Fragments/*GENETICS  Polymerase Chain Reaction  Sequence
       Homology, Amino Acid  Uganda  Variation (Genetics)  MEETING ABSTRACT

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