Document 3277
 DOCN  M94A3277
 TI    Patterns of amino acid substitutions in the third variable envelope
       region for HIV within a host.
 DT    9412
 AU    Gojobori T; Yamaguchi Y; National Institute of Genetics, Shizuoka,
       Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):112 (abstract no. PA0069). Unique
       Identifier : AIDSLINE ICA10/94369298
 AB    OBJECTIVE: The purpose of this study is to examine the patterns of amino
       acid changes in the third variable envelope (V3) region of HIV by
       phylogenetic methods. The substitution patterns obtained would be useful
       to develop effective vaccines. METHODS: We constructed phylogenetic
       trees by use of nucleotide sequences of HIV within a host. Then, we
       estimated the number of each kind of amino acid substitutions for the V3
       region. RESULTS: We found hypervariable sites in the V3 region. At these
       hypervariable sites, certain types of amino acid substitutions were
       dominantly favored by HIV. In particular, it is observed that most of
       amino acid substitutions were caused by single nucleotide substitutions
       at each codon. DISCUSSION AND CONCLUSIONS: The pattern of amino acid
       substitutions in the V3 region is dominantly determined by particular
       types of amino acid changes at the hypervariable sites. This feature can
       be utilized for future development of synthesized vaccines by predicting
       evolutionary direction of HIV.
 DE    Amino Acid Sequence  AIDS Vaccines/ISOLATION & PURIF  Codon/GENETICS
       Evolution  Human  HIV/*GENETICS/ISOLATION & PURIF  HIV Envelope Protein
       gp120/*GENETICS  HIV Infections/MICROBIOLOGY  Peptide
       Fragments/*GENETICS  Phylogeny  *Variation (Genetics)  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).