Document 3278
 DOCN  M94A3278
 TI    Diversity (macrophage-tropism) in the V3 loop region of HIV-1 gp120 from
       saliva.
 DT    9412
 AU    Kakizawa J; Ando S; Morishita Y; Nishimura S; Oka S; Mori S; Muller W;
       Ushijima H; Department of Microbiology, Institute of Public Health,
       Tokyo,; Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):112 (abstract no. PA0068). Unique
       Identifier : AIDSLINE ICA10/94369297
 AB    OBJECTIVE: To analyze the V3 region of HIV-1 gp120 from saliva and to
       consider the diversity of HIV-1 from the other organs. MATERIALS AND
       METHODS: The saliva and blood samples from HIV positive patients were
       collected and HIV DNAs and RNAs were extracted by glass powder. TA
       cloning substances of PCR or RT-PCR products with primers of the V3
       region were sequenced in several clones in each sample. The results were
       compared each other and standard strains. RESULTS: The sequences were
       more likely to those from macrophage-tropic cells (SF-162mc strain) than
       lymphocyte-tropic cells (MN strain). DISCUSSION: Saliva is easy to use
       for clinical diagnosis and for research. The sequence indicates that
       saliva would be meaningful to consider the pathogenesis of HIV
       infection.
 DE    Comparative Study  Human  HIV Envelope Protein gp120/*GENETICS  HIV
       Infections/MICROBIOLOGY  HIV-1/*GENETICS/*ISOLATION & PURIF
       Lymphocytes/MICROBIOLOGY  Macrophages/MICROBIOLOGY  Organ Specificity
       Peptide Fragments/*GENETICS  Polymerase Chain Reaction
       Saliva/*MICROBIOLOGY  *Variation (Genetics)  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).