Document 3280
 DOCN  M94A3280
 TI    AIDS pathogenesis: drug abuse, cytokines, and viral strain.
 DT    9412
 AU    Xin K; Shapshak P; Nagano I; Stewart R; Dept. of Psychiatry, Univ. of
       Miami Medical School, FL 33136.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):112 (abstract no. PA0065). Unique
       Identifier : AIDSLINE ICA10/94369295
 AB    OBJECTIVE. We are investigating the pathogenesis of AIDS due to HIV-1
       infection, drug abuse, and cytokines. METHODS. We utilize culture and
       molecular techniques (in situ hybridization and immuno-histochemistry to
       detect HIV and identify cell types in the central and peripheral nervous
       systems) and PCR, cloning, and DNA sequencing to characterize the
       sequence heterogeneity of HIV in blood, CSF, and brain. RESULTS. 1.
       possible accelerative effects of cocaine, alcohol, and cocaethylene on
       HIV replication in drug abusers; 2. not all cytokine production occurs
       in HIV-infected cells in brain or sensory ganglia; 3. cocultures of
       macrophages and astrocytes show accelerated HIV replication; 4. DNA
       sequencing in the HIV envelope gene (V1-V5) is being done directly from
       brain and phylogenetic relationships are being analyzed.
       DISCUSSION/CONCLUSIONS. Specific envelope amino acid sequences may be
       associated with neurological disease. Knowledge of the amino acid
       sequence heterogeneity of HIV will contribute to the development of
       vaccines against HIV.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS/*ETIOLOGY/
       MICROBIOLOGY  Astrocytes/MICROBIOLOGY  Brain/MICROBIOLOGY  Cells,
       Cultured  Cytokines/*PHYSIOLOGY  DNA, Viral/GENETICS/ISOLATION & PURIF
       Genes, env  Human  *HIV-1/GENETICS/ISOLATION & PURIF/PHYSIOLOGY
       Macrophages/MICROBIOLOGY  Substance Abuse/*COMPLICATIONS  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Virus Replication  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).