Document 3283
 DOCN  M94A3283
 TI    Characterisation of HIV-1 virus isolates involved in mother to child
       transmission.
 DT    9412
 AU    Narwa R; Roques P; Boussin F; Marce D; Dormont D; CRSSA/CEA,
       Fontenay-aux-Roses, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):111 (abstract no. PA0062). Unique
       Identifier : AIDSLINE ICA10/94369292
 AB    OBJECTIVE: We investigated the selection of particular subspecies of
       mother's human immunodeficiency virus type 1 (HIV-1) isolates during in
       utero transmission to the infant. METHODS: We characterized HIV-1
       isolates involved in mother to child transmission by analyzing cell
       tropism and V3, V4, V5 env gene sequences. Isolates were obtained by
       peripheral blood mononuclear cell (PBMC) cultures and amplification in
       cord blood cell cocultures. Eight couples of infected women/newborns and
       six mothers who did not infect their babies were investigated between
       the delivery and the seventh month of life. We assessed cell tropism
       with human purified lymphocytes and elutriated monocytes and with
       lymphoblastoid cells HUT78. Direct sequencing was performed through PCR
       assay specific for HIV-1 env gene in the V3 to V5 region with DNA
       extracted 1) from PBMC and 2) from cells of cultures used during isolate
       amplification. RESULTS: 1) We demonstrated that all isolates were a
       mixture of strongly related viruses and that all isolates seemed to be
       from the MN subtype. 2) No difference was observed in cell tropism
       between the isolates derived from the newborns, from their mother and
       from non transmitting mothers. 3) Molecular analysis of V3: the newborn
       isolates were closely related to the mother isolates and were selected
       among virus quasispecies of the mother. However we did not find any
       specific characteristics among the transmitted isolates. The size of the
       sample is on the way to be incrised and final results will be presented
       and discussed at the conference. CONCLUSION: Regarding our preliminary
       results, the specificity of the transmitted viruses may be due to the
       combination of multiple factors (cell tropism, immune response of the
       mother, viral load, placenta) which could not be reduced to a unique
       biological or molecular parameter.
 DE    DNA, Viral/BLOOD/GENETICS  Female  Fetal Blood/MICROBIOLOGY  Genes, env
       Human  HIV Infections/COMPLICATIONS/*MICROBIOLOGY/*TRANSMISSION
       HIV-1/CLASSIFICATION/GENETICS/*ISOLATION & PURIF  Infant, Newborn
       Leukocytes, Mononuclear/MICROBIOLOGY  *Maternal-Fetal Exchange
       Polymerase Chain Reaction  Pregnancy  Pregnancy Complications,
       Infectious/*MICROBIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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