Document 3285
 DOCN  M94A3285
 TI    Transportation system of the viral proteins in the HIV-infected cells.
 DT    9412
 AU    Takahashi I; Takama M; Ushijima K; Ozel M; Gelderblom H; Centr. Lab. EM.
       Teikyo Univ. Sch. Med. Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):110 (abstract no. PA0060). Unique
       Identifier : AIDSLINE ICA10/94369290
 AB    OBJECTIVE: Hitherto the morphological studies on the HIV had revealed
       only the high-electron dense materials just under the plasma membranes
       at budding sites. To distinguish hidden features of the viral proteins,
       combined-technique of immuno electron microscope (IEM) and detergent
       treatment was applied to the HIV-infected cells. METHODS: Two cell lines
       (TALL-1/KB-1 and Molt-4/HIV-1) were used. The samples for pre-embedded
       IEM were treated with 0.02% saponin. RESULTS: The viral proteins (gp120,
       p24, and p17) were visualized on the cytoskeletal net works. These
       proteins were synthesized by the attached ribosomes on rERs and
       cytoskeletons, and then transferred via cytoskeletons to the plasma
       membrane, where env protein (gp120) lifted up the membrane and then
       sticked into it to construct the viral envelope. gag proteins (p24/p17)
       were composed according to the arrangement of env protein. DISCUSSION
       AND CONCLUSIONS: Combined-technique of IEM and stereo observation was
       useful to reveal the distributions of unobserved viral proteins.
       Different from usual protein synthesis, the viral proteins of the HIV
       were synthesized on the attached ribosomes on rERs and cytoskeletons
       with no relations to ERs or Golgi apparatus. These results would also
       suggest several informations for other Retroviruses.
 DE    Biological Transport, Active  Cell Line  Cell Membrane/METABOLISM
       Cytoskeleton/METABOLISM  Endoplasmic Reticulum/METABOLISM  Gene
       Products, gag/METABOLISM  Human  HIV Antigens/METABOLISM  HIV Core
       Protein p24/METABOLISM  HIV Envelope Protein gp120/METABOLISM  HIV
       Infections/*METABOLISM/MICROBIOLOGY  HIV-1/*METABOLISM  Microscopy,
       Immunoelectron  Ribosomes/METABOLISM  Viral Proteins/*METABOLISM
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).