Document 3286
 DOCN  M94A3286
 TI    The cytoskeletal proteins cleaving activity of the recombinant HIV-1
       protease.
 DT    9412
 AU    Azuma R; Furuishi K; Saito A; Shinagawa H; Ikeda S; Shoji S; Dept. of
       Biochem., Fac. of Pharm. Sci., Kumamoto Univ., Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):110 (abstract no. PA0059). Unique
       Identifier : AIDSLINE ICA10/94369289
 AB    OBJECTIVE: The purpose of the present work was to isolate a HIV-1
       protease from HIV-1 protease expressing E. coli, and to determine its
       enzymatic activity for synthetic oligopeptides and nonviral protein
       substrates. METHODS: HIV-1 protease expressing E. coli was lysed,
       extracted, and partially purified by DEAE-Cellulofine and SP-Toyopearl
       column chromatography. The enzymatic cleaving activities for synthetic
       oligopeptides and proteins were determined by HPLC and SDS-PAGE,
       respectively. RESULTS AND DISCUSSION: The HIV-1 protease was separated
       from the HIV-1 protease expressing E. coli. It cleaved the Tyr-Pro bond
       of the various oligopeptides, and had a pH optimum at pH 5.5 for
       suc-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln. It was strongly inhibited by
       pepstatin A. The enzyme specifically hydrolyzed a Phe-Ala bond of
       alpha-actinin which is a component of cytoskeltal proteins. The enzyme
       may be important for penetration of the virus into the host cells in
       addition to the key enzyme in HIV-1 gag protein processing.
 DE    Amino Acid Sequence  Binding Sites  Cytoskeletal
       Proteins/GENETICS/*METABOLISM  Escherichia coli/GENETICS  Human
       Hydrogen-Ion Concentration  HIV Protease/GENETICS/*METABOLISM
       HIV-1/*ENZYMOLOGY/GENETICS  In Vitro  Molecular Sequence Data
       Oligopeptides/CHEMICAL SYNTHESIS/GENETICS/METABOLISM  Recombinant
       Proteins/GENETICS/METABOLISM  Substrate Specificity  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).