Document 3288
 DOCN  M94A3288
 TI    HIV-1 gp160 processing in furin-defective LoVo cells.
 DT    9412
 AU    Nagai Y; Ohnishi Y; Shioda T; Institute of Medical Science, University
       of Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):110 (abstract no. PA0057). Unique
       Identifier : AIDSLINE ICA10/94369287
 AB    Furin, a subtilisin-like mammalian endopprotease, is believed to be
       responsible for processing various proprotein precursors of cellular and
       viral origin including the gp160 of HIV-1, which share the consensus
       processing site motif, Arg-X-Lys/Arg-Arg, for protease recognition. To
       confirm and extend the concept that the gp160 is processed by furin, we
       used here a cell line, LoVo, which was recently demonstrated to be
       furin-defective. Unexpectedly, LoVo cells were found to process gp160 as
       efficiently as normal cell lines do, hence being able to fuse with
       CD4-expressing HeLa cells and to produce fully infectious virions. On
       the other hand, the same cell line was almost totally incapable of
       processing Newcastle disease virus (NDV) fusion glycoprotein with a
       similar oligobasic cleavage-recognition motif, providing a strong case
       of furin-mediated processing. Our present study thus raises a further
       need to search and identify the proteinases involved in HIV-1 gp160
       processing rather than supports the notion that furin is responsible.
 DE    Amino Acid Sequence  Binding Sites  Cell Fusion  Cell Line  Consensus
       Sequence  Gene Products, env/GENETICS/*METABOLISM  Hela Cells  Human
       HIV-1/*METABOLISM  Molecular Sequence Data  Newcastle Disease
       Virus/METABOLISM  Protein Precursors/GENETICS/*METABOLISM  Protein
       Processing, Post-Translational  Substrate Specificity
       Subtilisins/*METABOLISM  Viral Fusion Proteins/METABOLISM  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).