Document 3291
 DOCN  M94A3291
 TI    Functional analysis of HIV-1 Gag proteins.
 DT    9412
 AU    Kawamura M; Kitamura T; Ishimoto A; Adachi A; Institute for Virus
       Research, Kyoto University, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):11 (abstract no. 016A). Unique
       Identifier : AIDSLINE ICA10/94369284
 AB    OBJECTIVE: To study functions of HIV-1 Gag proteins, various in-frame
       gag gene mutants were constructed and their replication properties were
       examined. METHODS: Growth kinetics of mutant viruses in several cell
       lines were determined by RT assays. Defective steps of the mutants in
       the viral life cycle were monitored by single-round replication assays.
       RESULTS: Some of the mutant viruses replicated fairly well in some
       specific cell lines. In cell lines which did not support replication of
       the mutants, the defects of the viruses were localized to early
       infection phase in addition to late phase. CONCLUSIONS: Our results
       indicate that HIV-1 Gag proteins play an important functional role,
       through the interaction with a cell factor(s), at an early replication
       phase. Determination of the precise defective step of the mutant viruses
       is now in progress.
 DE    Cell Line  Gene Products, gag/*GENETICS  HIV Core Protein p24/PHYSIOLOGY
       HIV-1/GROWTH & DEVELOPMENT/*GENETICS  *Virus Replication  MEETING
       ABSTRACT

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