Document 3293
 DOCN  M94A3293
 TI    The role of the gag protein myristoylation in HIV-1 production.
 DT    9412
 AU    Furuishi K; Oh-ishi H; Misumi S; Matsuoka H; Ikeda S; Hantani Y;
       Ashizawa H; Shoji S; Dept. of Biochem., Fac. of Pharm. Sci., Kumamoto
       Univ., Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):109 (abstract no. PA0055). Unique
       Identifier : AIDSLINE ICA10/94369282
 AB    OBJECTIVE: The purpose of the studies is to clearly determine the
       protein myristoylation of the gag protein in HIV-1 infected cell lines,
       and to resolve the role of the gag protein myristoylation in virion
       particle formation. METHODS: HIV-1 producing cell lines (CEM/LAV-1,
       U937/IIIB, U937/RF, H9/MN, Molt-4/IIIB, U1, ACH-2) were maintained at 37
       degrees C in RPMI-1640 medium (10% FCS) in 5% CO2. The N-myristoylation
       of the gag protein was determined both by fluorography with [3H]myristic
       acid and by western immunoblotting analyses with anti N-myristoyl-Gly
       monoclonal antibody. The role of the N-myristoylation was resolved by
       fluorography, immunochemical and paper technological methods with
       N-myristoyl inhibitors or cross-linkers. RESULTS AND DISCUSSION: The
       N-myristoylation of the gag protein in HIV-1 producing cell lines was
       reconfirmed and its blockage by the myristoyl inhibitors resulted in
       depression of the product of HIV-1 progeny virus. The cross-linked
       proteins (p17gag-gp41 and p17gag-gp160) were clearly detected by western
       immunoblotting analyses after cocultivation of CEM/LAV-1 in the presence
       of cross-linking reagents. These results suggest that the N-myristoyl
       moiety of the gag protein may be important for maturation of infectious
       viruses.
 DE    Blotting, Western  Cell Line  Cross-Linking Reagents  Gene Products,
       env/METABOLISM  Gene Products, gag/CHEMISTRY/*METABOLISM  Human  HIV
       Antigens/METABOLISM  HIV Envelope Protein gp41/METABOLISM  HIV-1/*GROWTH
       & DEVELOPMENT/*METABOLISM  Myristic Acids/*METABOLISM  Protein
       Precursors/METABOLISM  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).