Document 3294
 DOCN  M94A3294
 TI    The expression of gag-pol and gag-pol-env sequences of HIV-1 by
       recombinant vaccinia viruses.
 DT    9412
 AU    Grigoriev VB; Gibadulin RA; Liendeman LF; Sazykin AY; D.I. Ivanovsky
       Institute of Virology, Rus. Acad. Med. Sci.; Moscow.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):109 (abstract no. PA0054). Unique
       Identifier : AIDSLINE ICA10/94369281
 AB    OBJECTIVE: To study assembly of virus like particle (VLP) in process of
       expression of gag-pol and gag-pol-env sequences we constructed
       recombinant vaccinia virus (VV) strains (RVV). METHODS: RVVs were
       constructed by insertion of the gag-pol and gag-pol-env sequences into
       VV and termed as RV-1 and RV-2. The expression of HIV-1 proteins by RVVs
       was analyzed by immunoblotting and electron microscopy. (EM) RESULTS:
       RV-1 synthesized p55, p41 and p24 polypeptides in cells. P17 was
       presented in minor quantaties. This strain didn't form VLP in CV-1 and
       Hep-2 cell cultures. RV-2 synthesized p160gag, p55gag, 3-4 proteins of
       p50-53gag, as well as p41gag ahd p17gag. P24 was present in minor
       quantaties. In CV-1 cells gag-proteins didn t form VLP, however in Hep-2
       cells muture and immuture VLP can be found by EM. In the cells are
       co-infected with RV-1 and RV-2 all above mentioned proteins and VLP were
       found. CONCLUSION: The formation of VLP by RV-2 depends on type of cell
       culture and level of synthesis of p17. In the cells are co-infected with
       two strains, each strain proteins are processed separetely from each
       other.
 DE    Cell Line  Gene Expression  Gene Products, env/BIOSYNTHESIS/GENETICS
       Gene Products, gag/BIOSYNTHESIS/GENETICS  Gene Products,
       pol/BIOSYNTHESIS/GENETICS  *Genes, env  *Genes, gag  *Genes, pol  Human
       HIV Antigens/BIOSYNTHESIS/GENETICS  HIV-1/GROWTH &
       DEVELOPMENT/*GENETICS/METABOLISM  Recombination, Genetic  Vaccinia
       Virus/GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).