Document 3297
 DOCN  M94A3297
 TI    Retroviral integration into highly repetitive Alu and HERV-K elements in
       13.1 KB human CD4 locus.
 DT    9412
 AU    Blinov VM; Zverev VV; Nedospasov SA; Institute for Viral Preparations,
       Moscow, Russia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):108 (abstract no. PA0050). Unique
       Identifier : AIDSLINE ICA10/94369278
 AB    Sequence of the human genomic fragment encompassing 13.1 kilobases from
       the human CD4 locus have been determined. This fragment included three
       exons and two untrons of CD4 gene. The genomic sequences were analyzed
       for the presence of repetitive elements and their classification is
       described. Within the sequenced fragment of CD4 gene we revealed
       fourteen Alu repeats, eight of the repeats being clastered in two loci.
       All Alu repeats are located in introns; eleven repeats have the
       counter-transcription orientation (antisense Alu elements) while three
       repeats are oriented in the direction of transcription. We localized the
       retrovirus-like element HERV-K in the first intron of the human CD4 gene
       in opposite orientation; hence all canonical regulatory elements of
       HERV-K are encoded on the minus strand relative to CD4 mRNA. The
       peculiar feature of HERV-K element in CD4 locus is the occurrence of Alu
       repeat wihin the element. Insertional Alu repeat is oppositely oriented
       and flanked by the direct repeats. The possible evolutionary mechanisms
       of origination of Alu insertions are discussed.
 DE    Antigens, CD4/*GENETICS  DNA, Antisense/GENETICS  Evolution  Exons
       Human  In Vitro  Introns  *Repetitive Sequences, Nucleic Acid
       Retroviridae/*GENETICS  RNA, Messenger/GENETICS  Virus
       Integration/*GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).