Document 3298
 DOCN  M94A3298
 TI    Proviral DNA in virions from primary isolates.
 DT    9412
 AU    Asjo B; Sommerfelt M; National Virus Center, University of Bergen,
       Norway.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):108 (abstract no. PA0052). Unique
       Identifier : AIDSLINE ICA10/94369277
 AB    OBJECTIVE: To determine whether proviral DNA is present in HIV virions
       of primary isolates grown in primary cells. MATERIALS AND METHODS:
       Patient isolates with slow/low and rapid/high type characteristics were
       used to infect PHA blasts. Virions were collected by ultracentrifugation
       and subjected to three different treatments prior to PCR amplification
       using primers hybridizing with U5 and R regions in the LTR (recognizing
       strong-stop DNA) and the pol region. Negative control consisted of
       pelleted disrupted virions treated with DNAse to remove adherent
       contaminating proviral DNA from disrupted cells. RESULTS: Proviral DNA
       corresponding to strong-stop DNA was demonstrated in virions from
       primary isolates of both slow/low and rapid/high phenotype. No signal
       could be detected with primers to the pol region which is in agreement
       with previous data that only partial reverse transcription takes place
       within the virion. CONCLUSION: Our data show that strong-stop DNA
       synthesis is not only a phenomenon associated with laboratory adapted
       strains of HIV but is also present in primary isolates grown in primary
       cells. It most likely represents a phenomenon common to the retrovirus
       family.
 DE    Cells, Cultured  Deoxyribonucleases  DNA,
       Viral/BIOSYNTHESIS/GENETICS/*ISOLATION & PURIF  Genes, pol  Human
       HIV/GENETICS/*ISOLATION & PURIF  HIV Long Terminal Repeat  Phenotype
       Polymerase Chain Reaction  Proviruses/GROWTH &
       DEVELOPMENT/GENETICS/*ISOLATION & PURIF  Virus Cultivation  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).