Document 3300
 DOCN  M94A3300
 TI    Effect of human interferon-induced dsRNA-dependent protein kinase on
       HIV-1 infection.
 DT    9412
 AU    Nagai K; Wainberg MA; Geleziunas R; Sonenberg N; Koromillas AE; McGill
       AIDS Centre, McGill University, Montreal, Qc, Canada.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):108 (abstract no. PA0051). Unique
       Identifier : AIDSLINE ICA10/94369275
 AB    The interferon (IFN)-induced dsRNA-dependent protein kinase (PKR) is a
       serine-threonine kinase that displays two distinct kinase activities: i)
       activation by autophosphorylation, and ii) phosphorylation of the
       alpha-subunit of eukaryotic initiation factor, elF-2, which causes
       inhibition of protein synthesis. PKR activation by specific virus RNAs
       has been suggested to play a key role in the IFN response to viral
       infection. Infection with human immunodeficiency virus 1 (HIV-1) is
       associated with the downregulation of PKR which is mediated by the
       virus-encoded regulatory protein, Tat. We have studied the role of PKR
       in HIV-1 production using the human monoblastoid cell line U-937 and
       have established and characterized cell lines expressing a wild-type
       (wt) human PKR or a dominant negative catalytically inactive PKR mutant
       (delta 6; deletion of LFIQME residues at position 361 to 366 of the
       human protein). We found that wt PKR-expressing U-937 cells that were
       transfected with the HXB2-D clone of HIV-1 produced viral products
       (reverse transcriptase and p24) more rapidly (7 days vs. 25 days) than
       did wild-type cells.
 DE    Amino Acid Sequence  Cell Line  Down-Regulation (Physiology)  Enzyme
       Induction/DRUG EFFECTS  Gene Expression  Human  HIV
       Infections/*ENZYMOLOGY  *HIV-1  Interferons/*PHARMACOLOGY  Molecular
       Sequence Data  Protein-Serine-Threonine Kinases/*BIOSYNTHESIS/GENETICS
       Sequence Deletion  Transfection  MEETING ABSTRACT

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