Document 3302
 DOCN  M94A3302
 TI    Requirement of host factors for HIV-1 Rev function.
 DT    9412
 AU    Shukla RR; Marquis SM; Kimmel PL; Kumar A; Department of Biochemistry &
       Molecular Biology, George Washington; University, Washington DC 20037.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):107 (abstract no. PA0047). Unique
       Identifier : AIDSLINE ICA10/94369273
 AB    The nucleocytoplasmic transport of unspliced or singly spliced viral
       mRNA transcripts is required for the expression of Human
       immunodeficiency virus type 1 structural proteins. HIV-1 regulatory
       protein Rev binds to Rev Responsive Element (RRE) in the Env encoding
       region of the viral mRNA and facilitates the transport of these
       transcripts. Interaction of RRE and Rev is essential but insufficient
       for Rev function since deletion of non Rev binding domains of RRE or non
       RRE binding domain of Rev results in a significant loss of Rev function.
       These observations suggest a requirement of host cell cofactors in Rev
       function. Recently we reported the formation of specific RRE RNA-host
       nuclear protein RNP complexes in an in vitro RNase protection gel
       retardation assay. Non-human cells such as CHO and A9 lack the ability
       to form these RNP complexes, however, the presence of human chromosome 6
       in A9 cells enabled these cells to form specific RNP complexes
       suggesting a role of human cofactor. We have confirmed these in vitro
       observations by in vivo analysis of Rev function using a CAT reporter
       construct, DM128. The construct DM128 contains CAT gene cloned in the
       Env encoding gene 5'to RRE and contains the authentic HIV-1 splice donor
       and acceptor sites. We show that mouse-human microcell hybrids
       containing human chromosome 6 or 11 support Rev function. These results
       demonstrate that human chromosomes 6 and 11 encode host cofactor
       required for Rev mediated transport of RRE containing HIV-1 mRNA. Rev
       function in the human chromosome 11 containing microcell hybrids
       suggests that host factors which interact with Rev or RRE independently
       of each other are required for optimal Rev function in vivo.
 DE    Animal  Cell Line  Chromosomes, Human, Pair 11  Chromosomes, Human, Pair
       6  CHO Cells/METABOLISM/MICROBIOLOGY  Gene Products,
       rev/*GENETICS/*PHYSIOLOGY  Genes, rev  Hamsters  Human  Hybrid
       Cells/METABOLISM/MICROBIOLOGY/ULTRASTRUCTURE
       HIV-1/*GENETICS/*PHYSIOLOGY  In Vitro  Ribonucleoproteins/METABOLISM
       RNA, Messenger/GENETICS  RNA, Viral/GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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