Document 3304
 DOCN  M94A3304
 TI    Cellular factor that binds to the leucine-rich motifs of HIV-1 Rev and
       HTLV-I Rex proteins.
 DT    9412
 AU    Kubota S; Adachi Y; Copeland TD; Oroszlan S; ABL-Basic Research Program,
       NCI-FCRDC, MD.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):107 (abstract no. PA0048). Unique
       Identifier : AIDSLINE ICA10/94369271
 AB    OBJECTIVE: HIV-I Rev protein and HTLV-I Rex protein possess their own
       conserved leucine-rich domains which are thought to interact with
       cellular factors. Our aim has been to isolate and characterize these
       co-factors via biochemical approaches. METHODS: Oligopeptides
       corresponding to the motifs of Rev (EPVPLQLPPLERLTLDCNED) and Rex
       (MDALSAQLYSSLSDSPP) were chemically synthesized and coupled to Sepharose
       4B resin by chemical cross-linking. The affinity resin was used to
       isolate cellular factors and to carry out various kinds of protein
       binding analysis. RESULTS: By affinity-chromatography of the whole cell
       extract from MOLT-4 cells with conjugated Rev-peptide, we partially
       purified a cellular protein of approximately 18KDa. Interestingly, the
       purified protein was able to interact not only with the Rev-derived
       peptide but also with the Rex specific peptide. DISCUSSION AND
       CONCLUSIONS: It was reported that eukaryotic initiation factor 5A
       (eIF-5A) is a cellular target for the leucine-rich domain of Rev, but
       the purified protein was no longer active in binding assays (Ruhl et
       al., J. Cell Biol. 123:1309, 1993). Studies are ongoing to characterize
       and determine the exact nature of the binding protein we have isolated
       and found to interact with both the Rev and Rex peptides.
 DE    Amino Acid Sequence  Binding Sites  Carrier Proteins/CHEMISTRY/ISOLATION
       & PURIF/METABOLISM  Cell Line  Gene Products, rev/GENETICS/*METABOLISM
       Gene Products, rex/GENETICS/*METABOLISM  Human
       HIV-1/GENETICS/*METABOLISM  Leucine/METABOLISM  Molecular Sequence Data
       Molecular Weight  Peptide Initiation Factors/METABOLISM  Support, U.S.
       Gov't, P.H.S.  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).