Document 3306
 DOCN  M94A3306
 TI    Role of natural suppressor glutamine tRNA on growth control of
       retroviruses.
 DT    9412
 AU    Kuchino Y; Muller WE; Odawara T; Yoshikura H; Biophys. Div., National
       Cancer Center Res. Inst., Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):106 (abstract no. PA0041). Unique
       Identifier : AIDSLINE ICA10/94369269
 AB    Frameshift suppression or nonsense suppression has a crucial role in
       expression regulation of retrovirus genomic informations. Recently, we
       isolated a natural suppressor tRNA from human and rodent cells, which
       recognizes the UAG amber codon as a glutamine codon in an in vitro
       translation system prepared from a rabbit reticulocyte lysate.
       Interestingly, the increase of the glutamine tRNA was dependent on
       retrovirus infection and subsequent vegetative proliferation of the
       virus such as MoMuLV and HIV-1. Therefore, it is imoportant to
       understand the molecular mechanism of expression regulation of the
       glutamine tRNA in mammalian cells for developing of the inhibition
       method of virus proliferation. Our recent investigations indicated that
       the mammalian gene encoding the suppressor glutamine tRNA commonly
       contained a unique signal sequence in the 5'-flanking region of the
       gene. In addition, we found that the binding of a nuclear protein to the
       sequence inhibited the transcription activity of the tRNA gene in vitro.
       We would like to discuss the transcriptional regulation of the
       suppressor glutamine tRNA gene in retrovirus infected cells.
 DE    Animal  Base Sequence  Codon/GENETICS  *Genes, Suppressor
       Glutamine/*GENETICS  Human  HIV-1/GROWTH &
       DEVELOPMENT/GENETICS/PHYSIOLOGY  In Vitro  Moloney Leukemia Virus/GROWTH
       & DEVELOPMENT/GENETICS/PHYSIOLOGY  Retroviridae/*GROWTH &
       DEVELOPMENT/*GENETICS/PHYSIOLOGY  Retroviridae Infections/MICROBIOLOGY
       RNA, Transfer/*GENETICS  Transcription, Genetic  Virus
       Replication/GENETICS  MEETING ABSTRACT

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