Document 3307
 DOCN  M94A3307
 TI    Regulation of HIV by retinoic acid (RA) correlates with expression of RA
       receptors.
 DT    9412
 AU    Yamaguchi K; Groopman JE; Byrn RA; Division of Hematology/Oncology, New
       England Deaconess Hospital,; Bostron, MA 02215.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):106 (abstract no. PA0042). Unique
       Identifier : AIDSLINE ICA10/94369268
 AB    OBJECTIVE: Analyze the effect of retinoic acids (RA) on human
       immunodeficiency virus type 1 (HIV-1) expression and correlate this
       effect with levels of retinoic acid receptor expression in T-lymphoid
       and monocytoid cell lines. METHODS: The effect of all-trans retinoic
       acid and 9-cis retinoic acid on HIV-1 production in T-lymphoid (H9,CEM)
       and monocytoid (U937,THP-1) cell lines was measured during acute and
       chronic infection. The expression levels of human retinoic acid receptor
       (hRAR alpha, the receptor for all-trans RA) and the human retinoid-X
       receptor (hRXR alpha, the receptor for 9-cis RA) were determined by
       Northern blot analysis. RESULTS: Both all-trans RA and 9-cis RA
       inhibited virus replication in HIV-1 IIIB infected monocytoid cells,
       both in the presence and absence of the co-stimulatory agent PMA. The
       retinoids had weak stimulatory effects, or no effects, on HIV production
       by T cell lines. HIV production by PMA-stimulated T cell lines was
       inhibited by these retinoids. 9-cis RA was generally more effective than
       all-trans RA in inhibiting HIV production and both agents in combination
       were generally more effective than the single agents alone. hRAR alpha
       was expressed in H9, U937 and THP-1 cells, but almost undetectable in
       CEM cells. hRXR alpha was significantly expressed in U937 and THP-1
       cells, weakly expressed in H9 cells and not detectable in CEM cells.
       After stimulation by PMA, hRXR alpha expression increased in H9 cells
       and U937 cells but not CEM cells. hRAR alpha expression was unchanged in
       H9 and CEM cells, and elevated in U937 cells, after PMA stimulation.
       DISCUSSION AND CONCLUSIONS: The effect of retinoic acids on HIV-1
       expression was cell type dependent and partially correlated with
       cellular expression of RA receptors. Endogenous, or exogenously
       administered, retinoic acids may have a significant role in HIV
       regulation.
 DE    Cell Line  Human  HIV-1/*DRUG EFFECTS/PHYSIOLOGY  Monocytes/DRUG
       EFFECTS/METABOLISM/MICROBIOLOGY  Receptors, Cytoplasmic and
       Nuclear/METABOLISM  Receptors, Retinoic Acid/*METABOLISM  Stereoisomers
       T-Lymphocytes/DRUG EFFECTS/METABOLISM/MICROBIOLOGY  Tetradecanoylphorbol
       Acetate/PHARMACOLOGY  Tretinoin/CHEMISTRY/METABOLISM/*PHARMACOLOGY
       Virus Replication/DRUG EFFECTS/PHYSIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).