Document 3311
 DOCN  M94A3311
 TI    Identification of EBV-transformed lymphocytes in HIV-infected
       lymphnodes.
 DT    9412
 AU    Zheng H; Mori S; Dept. of Pathol., Univ. of Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):105 (abstract no. PA0040). Unique
       Identifier : AIDSLINE ICA10/94369264
 AB    OBJECTIVE: Most of AIDS-related lymphomas (AIDS-ML) are infected with
       EBV. In order to investigate the origin of these neoplastic cells, we
       made an analysis using molecular biologic methods. METHOD: We examined
       35 cases of HIV-infected lymphnodes that were in PGL stage. For the
       demonstration of EBERs, we used in situ hybridization, and for the
       lymphocyte markers and EBV-produced oncoprotein LMP-1 (latent membrane
       protein 1). we used immunostaining method. 20 cases of HIV-negative
       lymphnodes were also studied as controls. RESULTS: EBV-encoded small
       RNAs (EBERs)-positive cells were found in 15 out of 35 cases. All 20
       cases of controls were negative for EBER. Immunostaining revealed many
       of the EBER-positive cells to be positive for LMP, and most of the
       EBER-positive cells to be in B cell lineage with CD20 and the remaining
       part were macrophage positive for CD68. DISCUSSION: We found that much
       increased number of EBV-bearing B cells, (and macrophages) are present
       in lymphnodes of HIV-infected patients. We believe that these
       EBV-positive B cells expressing EBERs and LMP-1 are the very precursor
       cells of AIDS-lymphomas.
 DE    Antigens, Viral/GENETICS/METABOLISM
       B-Lymphocytes/MICROBIOLOGY/PATHOLOGY  Herpesvirus 4,
       Human/GENETICS/IMMUNOLOGY/*ISOLATION & PURIF  Human  HIV
       Infections/*MICROBIOLOGY/PATHOLOGY  Lymph Nodes/*MICROBIOLOGY/PATHOLOGY
       Lymphoma, AIDS-Related/*MICROBIOLOGY/PATHOLOGY
       Macrophages/MICROBIOLOGY/PATHOLOGY  RNA, Viral/GENETICS/ISOLATION &
       PURIF  Tumor Stem Cells/MICROBIOLOGY/PATHOLOGY  Viral Matrix
       Proteins/GENETICS/METABOLISM  MEETING ABSTRACT

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