Document 3319
 DOCN  M94A3319
 TI    HIV-1 production from unintegrated provirus in transfected Jurkat
       clones.
 DT    9412
 AU    Gurgo C; Zella D; Reitz MS Jr; Gallo RC; Laboratory of Tumor Cell
       Biology, NCI NIH, Bethesda, MD.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):103 (abstract no. PA0030). Unique
       Identifier : AIDSLINE ICA10/94369256
 AB    Production of viral antigens and infectious virus by Jurkat cells
       transfected with pHXB2 (nef+) progressively declined over an extended
       period of time in culture. Sixty clones were generated from the
       transfected line; they were analyzed for the presence of provirus and
       monitored for the expression of viral antigens (surface gp120 and p24)
       and CD4. Six/60 clones were positive for the release of p24. Circular
       viral DNA was present in all p24 positive clones and absent in all p24
       negative clones (54/60). In addition to circular DNA, 5/6 p24+ clones
       contain an altered form of the original construct. The remaining p24+
       clone had the original pHXB2 gpt construct, as well as an altered form
       of the construct; it releases high levels of p24. All the p24- clones
       (54/60) contained altered forms of pHXB2 (but not the original
       construct). No clonally integrated provirus could be detected in any of
       the clones, suggesting that the clonal progenitor cell did not contain
       an integrated provirus. Thus, in the absence of integration, virus may
       persist in an unintegrated form, yet still be expressed and result in an
       active infection.
 DE    Cell Line  Clone Cells/MICROBIOLOGY  Genes, nef  Human  HIV
       Antigens/BIOSYNTHESIS  HIV Core Protein p24/BIOSYNTHESIS
       HIV-1/GENETICS/IMMUNOLOGY/*PHYSIOLOGY  Proviruses/GENETICS/*PHYSIOLOGY
       Transfection  Virus Integration/GENETICS/PHYSIOLOGY  Virus
       Replication/GENETICS/PHYSIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).