Document 3323
 DOCN  M94A3323
 TI    Identification of functional domains of HIV-1 integrase.
 DT    9412
 AU    Kitamura Y; Coffin JM; Division of Molecular Genetics, National
       Institute of Health,; Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):102 (abstract no. PA0026). Unique
       Identifier : AIDSLINE ICA10/94369252
 AB    Integration of a DNA copy of the viral RNA genome into host cellular DNA
       is essential and unique to the retroviral life cycle. Following reverse
       transcription, the 3' ends of the linear viral DNA are cleaved to expose
       the CA-OH ends (processing reaction) that become joined to the target
       DNA (strand transfer reaction). Purified integrase of several
       retroviruses including HIV have been shown to carry out both the 3' end
       processing and strand transfer reactions essential to integration. To
       identify functional domains of HIV-1 integrase, we constructed several
       chimera integrase genes; chimeras between HIV-1 and avian leukosis virus
       (ALV). Then, those mutants genes were expressed in E. coli and purified.
       When C-terminal half of the ALV integrase (just after DX35E domain) was
       replaced with that of HIV-1, the mutant failed to carry out both the end
       processing and strand transfer reactions. Although this region is
       supposed to be a simple (non-specific) target DNA-binding domain, the
       result implies that the region may have a role in retain a functional
       structure and configuration of integrase protein in addition to
       DNA-binding function. Result of integration activity assay of another
       HIV-1 mutant, whose N-terminal 'HHCC' domain was replaced with that of
       ALV, will also presented.
 DE    Binding Sites  Cloning, Molecular  DNA
       Nucleotidyltransferases/GENETICS/*METABOLISM  DNA-Binding
       Proteins/GENETICS/METABOLISM  Escherichia coli/GENETICS  Genes, Viral
       Human  HIV-1/*ENZYMOLOGY/GENETICS  In Vitro  Leukosis Virus,
       Avian/ENZYMOLOGY/GENETICS  Mutagenesis  Recombinant Fusion
       Proteins/GENETICS/METABOLISM  Virus Integration/GENETICS/PHYSIOLOGY
       MEETING ABSTRACT

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