Document 3324
 DOCN  M94A3324
 TI    Unessential of calcium ions and inhibitor specificity for the
       intracellular gp160 processing.
 DT    9412
 AU    Okumura Y; Kamoshita K; Koga Y; Sasaki M; Kido H; Inst. for Enz. Res.,
       Univ. of Tokushima, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):101 (abstract no. PA0024). Unique
       Identifier : AIDSLINE ICA10/94369251
 AB    OBJECTIVES: Endoproteolytic cleavage of the envelope glycoprotein
       precursor (gp160) of HIV-1 by cellular protease(s) is required for the
       full activity of virus. In this study, the requirement of calcium ions
       and the inhibitor specificity for the intracellular gp160 processing
       were analyzed. METHOD: Intracellular gp160 processing was analyzed in
       cultured HeLa cells and Molt-4, clone 8 cells which infected with
       recombinant vaccinia virus encoded gp160 gene. RESULTS AND CONCLUSIONS:
       Intracellular calcium ions depletion by 10(-6)M of EGTA, or EDTA or by
       10(-7)M of calcium-ionophore, A23187 in the calcium free medium and
       intracellular calcium ions administration by incubation with A23187 and
       2mM of CaCl2 had no effect on the gp160 processing in cultured HeLa
       cells and Molt-4, clone 8 cells. The gp160 processing in these cells was
       inhibited by substrate analog dec-RVKR-cmk, thiol reagents, heavy metal
       ions and low molecular weight serine protease inhibitor diisopropyl
       fluorophosphate. The results suggest that intracellular gp160 processing
       enzyme in these cells is not a member of Furin-like proteases and that
       the processing protease has serine and cysteine residues in the active
       site region.
 DE    Amino Acid Sequence  Binding Sites  Calcimycin/PHARMACOLOGY
       Calcium/*METABOLISM  Cell Line  Edetic Acid/PHARMACOLOGY  Egtazic
       Acid/PHARMACOLOGY  Gene Products, env/GENETICS/*METABOLISM  Hela Cells
       Human  HIV-1/DRUG EFFECTS/*METABOLISM  Molecular Sequence Data
       Oligopeptides/CHEMISTRY/PHARMACOLOGY  Peptide
       Peptidohydrolases/METABOLISM  Protein Precursors/GENETICS/*METABOLISM
       Protein Processing, Post-Translational/DRUG EFFECTS  Recombinant
       Proteins/GENETICS/METABOLISM  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).