TI:  Can Ozone Administration activate the Tissue Metabolism?  A 
     Study on Brain Metabolism During Hypoxic Hypoxia

DT:  Jan 1993

AU:  Shiratori R., Kaneko Y., Kobayashi Y., Yamomoto Y., Sano H., 
     Ishizu Y., Yamamoto T.
     Department of Anesthesiology, Nihon University School of 
     Medicine, Tokyo

SO:  Masui - Japanese Journal of Anesthesiology 42(1):2-6, 1993 
     Jan.

AB:  As it was thought that the tissue metabolism could be 
     activated by administration of ozone, the influence of ozone 
     on cerebral metabolism was studied under hypoxic condition.  
     Ozone 1.5% was obtained from an ozonizer which separates the 
     ozone from oxygen.  Objects were 30 mature rabbits and they 
     inhaled 3% oxygen in 97% nitrogen (PaO2 17-19 mmHg) by 
     spontaneous respiration through a tracheostomy cannula.  
     Objects were divided into three groups.  Control rabbits 
     inhaled only anoxic gases without ozone administration.  
     Although the oxygen group also inhaled anoxic gases, pure 
     oxygen was administered through an A-V shunt, previously 
     made on femoral artery and vein, with an oxygenator, named 
     Nichidai style (counter-current-exchange method).  
     Similarly, the ozone group was given ozone with the 
     oxygenator via A-V shunt.  In each group, survival time was 
     well as ATP, ADP, AMP and lactic acid lipo-peroxide in brain 
     were examined.  After the administration of ozone, survival 
     time was prolonged significantly, compared with the control 
     group (P < 0.005).  Equally, ATP and energy charge in the 
     brain were well maintained (P < 0.005) and the lactate 
     production was inhibited significantly (P < 0.005).  These 
     results indicate that oxidizing effect of ozone will be 
     beneficial to maintain energy charge in brain tissue during 
     hypoxia.  Further, ozone might activate the energy 
     metabolism in animal tissues.