Document 0109
 DOCN  M9440109
 TI    Establishment of HTLV-I-infected cell lines from French, Guianese and
       West Indian patients and isolation of a proviral clone producing viral
       particles.
 DT    9404
 AU    Nicot C; Astier-Gin T; Edouard E; Legrand E; Moynet D; Vital A;
       Londos-Gagliardi D; Moreau JP; Guillemain B; INSERM, Unite 328,
       Bordeaux, France.
 SO    Virus Res. 1993 Dec;30(3):317-34. Unique Identifier : AIDSLINE
       MED/94152160
 AB    Human T-cell leukemia virus (HTLV-I) induces adult T cell
       leukemia/lymphoma (ATL) and a chronic neurological disease named either
       tropical spastic paraparesis (TSP) or HTLV-I associated myelopathy
       (HAM). We report here the establishment and characterization of eight
       HTLV-I-infected lymphoid cell lines derived either from patients with
       TSP (5) or from asymptomatic carriers (1). Southern blot analysis of T
       cell beta chain gene rearrangements indicates that all cell lines are
       composed of clonal populations. The same type of analysis performed with
       HTLV-I-specific probes showed that they harbor 1 to 5 copies of full
       length proviruses often associated with deleted proviruses with a
       restriction map for BamHI, HindIII, PstI and SacI restriction enzymes
       resembling those of HTLV-I previously isolated from Japan and Caribbean
       area. One of the cell lines, 2060, derived from a TSP patient was shown
       to express a relative large amount of virus easily transmissible to
       fresh peripheral and cord blood lymphocytes. The full length proviral
       genome contained in this cell line was cloned and used in transient
       expression experiments. We showed that the cloned provirus was able to
       direct the synthesis of the major structural viral proteins, the
       protease and the tax and rex regulatory proteins. The structural viral
       proteins could be assembled into free particles detected in the culture
       medium of transfected cells. Although the infectivity of these viral
       particles remains to be determined, this new clone can be employed to
       examine the cell types in which this TSP-derived provirus directs viral
       protein synthesis and eventually replicates. It should also prove of
       value in studies on the early cellular events induced by viral products.
 DE    Animal  Base Sequence  Cell Line  Cercopithecus aethiops  Cloning,
       Molecular  DNA, Viral/GENETICS  French Guiana  Gene Expression  Genes,
       env  Human  *HTLV-I/GENETICS/ISOLATION & PURIF/PHYSIOLOGY  HTLV-I
       Infections/*MICROBIOLOGY  Martinique  Microscopy, Electron  Molecular
       Sequence Data  Proviruses/GENETICS/ISOLATION & PURIF/PHYSIOLOGY
       Support, Non-U.S. Gov't  Virus Integration  Virus Replication  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).