Document 0136
 DOCN  M9440136
 TI    Induction of RNA-binding proteins in mammalian cells by DNA-damaging
       agents.
 DT    9404
 AU    Carrier F; Gatignol A; Hollander MC; Jeang KT; Fornace AJ Jr; Laboratory
       of Molecular Pharmacology, National Cancer Institute,; NIH, Bethesda, MD
       20892.
 SO    Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1554-8. Unique Identifier :
       AIDSLINE MED/94151368
 AB    A technique to detect RNA-binding proteins (RBP) involving hybridization
       of RNA probe to proteins transferred to a membrane was used to study RBP
       in different mammalian cells and in cells after genotoxic stress. With
       this approach, up to 13 proteins of different sizes were detected in
       crude nuclear extracts by using a viral RNA probe consisting of the
       trans-activation-responsive (TAR) element of human immunodeficiency
       virus type 1 (HIV-1). The TAR RNA probe contains a stem-loop structure
       found in nascent HIV-1 transcripts. A G+C-rich probe with similar
       structure also bound to many of these RBP. Only a 102-kDa protein
       strongly bound to other RNA probes lacking this structure, while a probe
       with an A+U-rich stem-loop structure fail to bind most RBP, thus
       indicating a RNA secondary structure preference. The expression of these
       RBP varied substantially in nine different human and hamster cell lines,
       with no detectable RBP in two human myeloid lines. Evidence for
       induction of these RBP was found in six of seven lines after treatment
       with DNA-damaging agents; UV radiation was the most effective agent. In
       Chinese hamster ovary cells, which showed the strongest response, all
       five RBP present in untreated cells rapidly increased in activity after
       UV irradiation, and eight additional RBP were detected. The induction of
       these RBP by DNA-damaging agents indicates one or more possible roles
       for these proteins in the cellular response to genotoxic stress and in
       viral activation after such stress.
 DE    Animal  Cell Line  Comparative Study  CHO Cells/RADIATION EFFECTS  DNA
       Damage/*PHYSIOLOGY  Gamma Rays  *Gene Expression Regulation  Hamsters
       Human  Methyl Methanesulfonate/PHARMACOLOGY  Nuclear Proteins/METABOLISM
       Protein Binding  RNA/METABOLISM  RNA-Binding Proteins/*METABOLISM
       Species Specificity  Ultraviolet Rays  Xeroderma Pigmentosum/METABOLISM
       JOURNAL ARTICLE

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