Document 0138
 DOCN  M9440138
 TI    Human immunodeficiency virus type 1 Nef associates with a cellular
       serine kinase in T lymphocytes.
 DT    9404
 AU    Sawai ET; Baur A; Struble H; Peterlin BM; Levy JA; Cheng-Mayer C;
       Department of Medicine, University of California, San Francisco;
       94143-0128.
 SO    Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1539-43. Unique Identifier :
       AIDSLINE MED/94151365
 AB    With T-cell lines constitutively expressing Nef from the SF2 strain of
       human immunodeficiency virus type 1 (HIV-1SF2) in the form of a hybrid
       CD8-Nef fusion protein or T-cell lines chronically infected with
       HIV-1SF2, a cellular serine kinase was found that specifically
       associates with Nef. Proteins of 62 kDa and 72 kDa, which
       coimmunoprecipitated with Nef, were phosphorylated in in vitro kinase
       assays. This Nef-associated serine kinase activity was not blocked by
       inhibitors of protein kinase C or protein kinase A and was lost when Nef
       was truncated at amino acid 94 or 99. These findings present evidence
       that a serine kinase activity is associated with Nef expressed in human
       T lymphocytes.
 DE    Antigens, CD8/GENETICS/METABOLISM  Cells, Cultured  Chimeric
       Proteins/METABOLISM  Cyclic AMP-Dependent Protein Kinases/ANTAGONISTS &
       INHIB  DNA Mutational Analysis  Gene Products, nef/GENETICS/*METABOLISM
       Human  HIV-1/*METABOLISM  Macromolecular Systems  Phosphoserine/ANALYSIS
       Precipitin Tests  Protein Binding  Protein Kinase C/ANTAGONISTS & INHIB
       Protein-Serine-Threonine Kinases/*METABOLISM  Sequence Deletion
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/*MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).