Document 0139
 DOCN  M9440139
 TI    Analysis of the T-cell receptor beta-chain variable-region (V beta)
       repertoire in monozygotic twins discordant for human immunodeficiency
       virus: evidence for perturbations of specific V beta segments in CD4+ T
       cells of the virus-positive twins.
 DT    9404
 AU    Rebai N; Pantaleo G; Demarest JF; Ciurli C; Soudeyns H; Adelsberger JW;
       Vaccarezza M; Walker RE; Sekaly RP; Fauci AS; Laboratory of
       Immunoregulation, National Institute of Allergy and; Infectious
       Diseases, National Institutes of Health, Bethesda, MD; 20892.
 SO    Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1529-33. Unique Identifier :
       AIDSLINE MED/94151363
 AB    We analyzed the T-cell receptor (TCR) V beta repertoire in human
       immunodeficiency virus type 1 (HIV-1)-infected individuals at different
       stages of disease. To circumvent the effect of HLA and other loci on the
       expressed TCR repertoire, we compared the TCR repertoire in nine pairs
       of monozygotic twins who were discordant for HIV infection. A
       semiquantitative polymerase chain reaction (PCR) assay and flow
       cytometry enabled us to show distinct differences in the V beta
       repertoire in the HIV-positive twin compared with the HIV-negative twin.
       By combining PCR and cytofluorometry, these differences were restricted
       to a specific set of TCR V beta segments, with members of the V beta 13
       family perturbed in six out of seven cases and those of the V beta 21
       family perturbed in four out of seven cases studied. Most of the other V
       beta families remained unchanged. Our results provide direct evidence
       for a skewed TCR repertoire in HIV infection.
 DE    Antibodies, Monoclonal  Antigens, CD8/IMMUNOLOGY  Comparative Study
       Flow Cytometry  Human  HIV Infections/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       Leukapheresis  Polymerase Chain Reaction  Receptors, Antigen, T-Cell,
       alpha-beta/*GENETICS  Superantigens/IMMUNOLOGY  Support, Non-U.S. Gov't
       T-Lymphocyte Subsets/*IMMUNOLOGY  Twins, Monozygotic  T4
       Lymphocytes/IMMUNOLOGY  *Variation (Genetics)  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).