Document 0141
 DOCN  M9440141
 TI    A small element from the Mason-Pfizer monkey virus genome makes human
       immunodeficiency virus type 1 expression and replication
       Rev-independent.
 DT    9404
 AU    Bray M; Prasad S; Dubay JW; Hunter E; Jeang KT; Rekosh D; Hammarskjold
       ML; Myles H. Thaler Center for AIDS and Human Retrovirus Research,;
       University of Virginia, Charlottesville 22908.
 SO    Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1256-60. Unique Identifier :
       AIDSLINE MED/94151308
 AB    Replication of human immunodeficiency virus type 1 (HIV-1) is dependent
       on the viral Rev protein. This protein acts in concert with the
       cis-acting rev-responsive element present in intron-containing RNAs to
       facilitate nuclear export of these RNAs. Here we show that a cis-acting
       219-nucleotide sequence from an unrelated simple retrovirus,
       Mason-Pfizer monkey virus (MPMV), enables Rev-independent HIV-1
       replication. This sequence is present in an untranslated region near the
       3' end of the MPMV genome. The MPMV element is also able to efficiently
       substitute for Rev in expression of Gag/Pol and Env proteins from
       subgenomic constructs. We hypothesize that the MPMV element functions by
       interacting with a cellular factor that plays a role in mRNA transport
       analogous to that of the Rev protein. It might be possible to exploit
       this element in the development of an HIV vaccine.
 DE    Biological Transport  Cells, Cultured  DNA Mutational Analysis  Fusion
       Proteins, gag-pol/BIOSYNTHESIS  Gene Products, env/BIOSYNTHESIS  Gene
       Products, gag/BIOSYNTHESIS  Gene Products,
       rev/*DEFICIENCY/GENETICS/METABOLISM  Genome, Viral  HIV-1/*GROWTH &
       DEVELOPMENT  Mason-Pfizer Monkey Virus/*GENETICS  Polarity of
       Translation  Regulatory Sequences, Nucleic Acid/*GENETICS  RNA,
       Messenger/METABOLISM  RNA, Viral/METABOLISM  Sequence Deletion  Sequence
       Homology, Nucleic Acid  Support, U.S. Gov't, P.H.S.  Virus Replication
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).