Document 0183
 DOCN  M9440183
 TI    Reduced nuclear import of human immunodeficiency virus type 1
       preintegration complexes in the presence of a prototypic nuclear
       targeting signal.
 DT    9404
 AU    Gulizia J; Dempsey MP; Sharova N; Bukrinsky MI; Spitz L; Goldfarb D;
       Stevenson M; Department of Pathology and Microbiology, University of
       Nebraska; Medical Center, Omaha 68198-5120.
 SO    J Virol. 1994 Mar;68(3):2021-5. Unique Identifier : AIDSLINE
       MED/94149903
 AB    Nuclear import of the retroviral preintegration complex and integration
       of retroviral with host cell DNA are essential steps for completion of
       the virus life cycle. The preintegration complex of the lentivirus human
       immunodeficiency virus type 1 (HIV-1) displays karyophilic properties
       and, as a consequence, is rapidly directed to the host cell nucleus by
       an energy-dependent transport pathway. The karyophilic properties of
       nuclear proteins are governed by a nuclear localization sequence, the
       targeting function of which can be inhibited in the presence of excess
       targeting signals. Here we present evidence that the nuclear import of a
       large karyophile--the preintegration complex of HIV-1--is inhibited in
       the presence of a prototypic nuclear targeting signal of simian virus 40
       T antigen. This points to a novel strategy which prevents establishment
       of the provirus by interrupting nuclear localization of HIV-1 DNA.
 DE    Amino Acid Sequence  Antigens, Viral, Tumor/PHARMACOLOGY  Biological
       Transport/DRUG EFFECTS  Cell Compartmentation/*PHYSIOLOGY  Cell
       Nucleus/METABOLISM  Cytoplasm/METABOLISM  DNA, Circular/METABOLISM  DNA,
       Complementary/METABOLISM  DNA, Viral/*METABOLISM  HIV-1/*METABOLISM
       Models, Genetic  Molecular Sequence Data  Peptide Fragments/PHARMACOLOGY
       Signal Peptides/*PHYSIOLOGY  Support, U.S. Gov't, P.H.S.  Virus
       Integration/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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