Document 0185
 DOCN  M9440185
 TI    Functional role of the zipper motif region of human immunodeficiency
       virus type 1 transmembrane protein gp41.
 DT    9404
 AU    Chen SS; Department of Pediatrics, Harvard Medical School, Boston,;
       Massachusetts.
 SO    J Virol. 1994 Mar;68(3):2002-10. Unique Identifier : AIDSLINE
       MED/94149900
 AB    To study the functional role of the zipper motif region, located in the
       N-terminal region of the envelope transmembrane protein of human
       immunodeficiency virus type 1, a series of vaccinia virus-expressed
       mutant proteins containing a proline substitution in this region were
       characterized. All of the mutant proteins showed partial or no
       inhibition in gp160 cleavage, demonstrated impaired ability of gp120 to
       associate with gp41, and were unable to mediate syncytium formation with
       CD4+ cells. Moreover, mutants 580 and 587 secreted excessive gp120 into
       the medium compared with the wild type. Mutations in this region
       affected the conformation of the local or proximal sequence but did not
       alter the conformation conferred by a distal site. These studies reveal
       the crucial role of the C-terminal segment of the zipper motif region in
       envelope heterodimeric association and suggest that this sequence forms
       a gp120 contact site.
 DE    Amino Acid Sequence  Antibodies, Monoclonal  Antibodies, Viral
       Antigenic Determinants  Antigens, CD4/METABOLISM  Base Sequence  Cell
       Fusion  Comparative Study  DNA Mutational Analysis  Gene Products,
       env/METABOLISM  HIV Envelope Protein gp120/METABOLISM  HIV Envelope
       Protein gp41/GENETICS/IMMUNOLOGY/*METABOLISM  HIV-1/*PHYSIOLOGY  Leucine
       Zippers/*PHYSIOLOGY  Molecular Sequence Data  Protein Binding  Protein
       Precursors/METABOLISM  Protein Processing, Post-Translational  Support,
       Non-U.S. Gov't  Vaccinia Virus/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).