Document 0193
 DOCN  M9440193
 TI    Enhanced in vitro human immunodeficiency virus type 1 replication in B
       cells expressing surface antibody to the TM Env protein.
 DT    9404
 AU    Tani Y; Donoghue E; Sharpe S; Boone E; Lane HC; Zolla-Pazner S; Cohen
       DI; Laboratory of Immunoregulation, National Institute of Allergy and;
       Infectious Diseases, Bethesda, Maryland 20892.
 SO    J Virol. 1994 Mar;68(3):1942-50. Unique Identifier : AIDSLINE
       MED/94149889
 AB    The human immunodeficiency virus type 1 (HIV-1) external envelope
       glycoprotein gp120 tightly binds CD4 as its principal cellular receptor,
       explaining the tropism of HIV-1 for CD4+ cells. Nevertheless, reports
       documenting HIV infection or HIV binding in cells lacking CD4 surface
       expression have raised the possibility that cellular receptors in
       addition to CD4 may interact with HIV envelope. Moreover, the lymphocyte
       adhesion molecule LFA-1 appears to play an important role in augmenting
       HIV-1 viral spread and cytopathicity in vitro, although the mechanism of
       this function is still not completely defined. In the course of
       characterizing a human anti-HIV gp41 monoclonal antibody, we transfected
       a CD4-negative, LFA-1-negative B-cell line to express an anti-gp41
       immunoglobulin receptor (surface immunoglobulin [sIg]/gp41). Despite
       acquiring the ability to bind HIV envelope, such transfected B cells
       could not be infected by HIV-1. These cells were not intrinsically
       defective for supporting HIV-1 infection, because when directed to
       produce surface CD4 by using retroviral constructs, they acquired the
       ability to replicate HIV-1. Interestingly, transfected cells expressing
       both surface CD4 and sIg/gp41 receptors replicated HIV much better than
       cells expressing only CD4. The enhancement resided specifically in
       sIg/gp41, because isotype-specific, anti-IgG1 antibodies directed
       against sIg/gp41 blocked the enhancement. These data directly establish
       the ability of a cell surface anti-gp41 receptor to enhance HIV-1
       replication.
 DE    Amino Acid Sequence  Antigens, CD4/*METABOLISM
       B-Lymphocytes/*IMMUNOLOGY/MICROBIOLOGY  Base Sequence  Gene Products,
       env/METABOLISM  Human  HIV Envelope Protein gp120/METABOLISM  HIV
       Envelope Protein gp41/*IMMUNOLOGY  HIV-1/*GROWTH & DEVELOPMENT
       Immunoglobulins, Surface/GENETICS/*METABOLISM  Molecular Sequence Data
       Protein Binding  Protein Precursors/METABOLISM  Receptors,
       HIV/GENETICS/*METABOLISM  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).